Microsatellite markers in coast redwood (Sequoia sempervirens)

被引:8
作者
Bruno, D [1 ]
Brinegar, C [1 ]
机构
[1] San Jose State Univ, Conservat Genet Lab, Dept Biol Sci, San Jose, CA 95192 USA
来源
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 03期
关键词
coast redwood; microsatellite markers; Sequoia sempervirens; simple sequence repeat;
D O I
10.1111/j.1471-8286.2004.00720.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Five polymorphic microsatellite markers are described for coast redwood, a hexaploid conifer. Dinucleotide (three), trinucleotide (one) and tetranucleotide (one) repeat loci were isolated from a genomic plasmid library and amplified in a test population of 25 second-growth redwoods. There were three to 11 alleles per locus and observed heterozygosities ranged from 0.00 to 0.80. The nonheterozygous locus (a CTTA repeat) is probably of chloroplast origin and may have potential as a marker for paternal inheritance.
引用
收藏
页码:482 / 484
页数:3
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