Novel Synthesis and Biological Evaluations of N,N'-disubstituted-3,6-bis(substitutedphenyl)-1,2,4,5-tetrazine-1,4-dicarboxamide

A series of novel N,N'-disubstituted-3,6-bis(substitutedphenyl) -1,2,4,5-tetrazine-1,4-dicarboxamides were prepared using the intermolecular cyclization reaction of N-substituted-N'-(alpha-chloro-substitutedbenzylidene) hydrazinecarboxamide and triethylamine by the modification of solvent polarity. The structures of all the new compounds were characterized by IR, H-1-NMR, MS and elemental analysis. Their cytostatic effects were screened in vitro by the SRB method for A-549 cell and the MTT method for P-388 cell. The results showed that several compounds demonstrate potential antitumor activities against P-388. The substituents have clearly effect on their antitumor activity.