Conventional and molecular investigation of meningococcal isolates in relation to two outbreaks in the area of Athens, Greece

被引：13

作者：

Tzanakaki, G.

Kesanopoulos, K.

Yazdankhah, S. P.

Levidiotou, S.

Kremastinou, J.

Caugant, D. A.

机构：

[1] Natl Sch Publ Hlth, Natl Meningitis Reference Lab, Athens, Greece

[2] Norwegian Inst Publ Hlth, Dept Bacteriol & Immunol, Div Infect Dis Control, Oslo, Norway

[3] Univ Ioannina, Sch Med, Dept Microbiol, GR-45110 Ioannina, Greece

[4] Univ Oslo, Inst Oral Biol, Oslo, Norway

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2006年 / 12卷 / 10期

关键词：

multilocus sequence typing; Neisseria meningtidis; outbreaks; random amplified polymorphic DNA; typing; variable number tandem repeats;

D O I：

10.1111/j.1469-0691.2006.01521.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Two local outbreaks caused by serogroup B Neisseria meningitidis occurred in the Athens area of Greece during 2003. In total, 30 N. meningitidis isolates from patients and carriers, as well as sporadic cases, were investigated by conventional techniques (serogroup, serotype and serosubtype), multilocus sequence typing (MLST), analysis of variable number tandem repeats (VNTR) and random amplified polymorphic DNA (RAPD) analysis. Compared with the two other molecular techniques, VNTR analysis was a simple, reliable and highly discriminatory method for fine typing of meningococcal isolates, showing a good correlation with the epidemiological data for the two outbreaks analysed.

引用

页码：1024 / 1026

页数：3

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