Selective amperometric sensing of hydrogen peroxide with Nafion/copper particulates chemically modified electrode

Nafion/copper particulates chemically modified glassy carbon electrode has been prepared by in situ electrochemical deposition. The electrode is found to to be successful for selective amperometric sensing of hydrogen peroxide at -0.2 V vs Ag/AgCl at physiological pH without interference from uric acid, ascorbic acid, catechol, cysteine, nitrite and nitrate. Two linear regions, 20-200 mu M and 220-400 mu M, for H(2)O(2) detection with the slope and regression coefficient values of 33.2 nA/mu M and 25.61 nA/mu M and 0.9975 and 0.9999 respectively are obtained with 1.63 mu M detection limit (S/N = 3). Application to real samples analysis is demonstrated for milk, urine and tea samples with appreciable recovery values.