Hydrothermal assisted Eichhornia crassipes mediated synthesis of magnetite nanoparticles (E-Fe3O4) and its antibiofilm activity

In this present study, we report for the first time, a hydrothermal assisted green synthesized magnetite nanoparticle (E-Fe3O4 NPs) using Eichhornia crassipes extract as reducing agent and its antibiofilm potential. The synthesized nanoparticles were characterized by x-ray diffraction (XRD), Transmission electron microscopy (TEM) analysis, Energy-Dispersive Spectrometer (EDS), Fourier transform infrared spectroscopy (FTIR), Zeta potential, Dynamic light scattering (DLS) studies and vibrating sample magnetometer (VSM). The E-Fe(3)O(4 )NPs were mostly spherical in shape with the average particle size of 10 +/- 4 nm and show long time stability and positive surface potential. FTIR spectral analysis confirmed the reduction, capping and the formation of E-Fe3O4 NPs. Further, on treating E-Fe3O4 NPs against bacterial strains (E. coli and S. aureus), it resulted antibacterial activity and inhibited biofilm formation. FE-SEM and CLSM results provide the visual evidence that E-Fe3O4 NPs arrested the bacterial growth and prevent biofilm formation in both the bacterial strains. In conclusion, the present study suggests that E-Fe3O4 NPs can play a key role in biological significance of the surface coating of biomaterials and for treatment in contrast to infections caused by highly antibiotic resistant biofilm producing bacteria.