Integrated photoacoustic, confocal, and two-photon microscope

被引：40

作者：

Rao, Bin ^{[1
]}

Soto, Florentina ^{[2
]}

Kerschensteiner, Daniel ^{[2
]}

Wang, Lihong V. ^{[1
]}

机构：

[1] Washington Univ, Opt Imaging Lab, Dept Biomed Engn, St Louis, MO 63130 USA

[2] Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2014年 / 19卷 / 03期

基金：

美国国家卫生研究院;

关键词：

confocal microscopy; two-photon microscopy; photoacoustic microscopy; optical-resolution photoacoustic microscopy; GREEN FLUORESCENT PROTEIN;

D O I：

10.1117/1.JBO.19.3.036002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine. However, exogenous fluorescence contrast agents may affect cellular structure and function, and fluorescence microscopy cannot image nonfluorescent chromophores. We overcome this limitation by integrating optical-resolution photoacoustic microscopy into a modern Olympus IX81 confocal, two-photon, fluorescence microscope setup to provide complementary, label-free, optical absorption contrast. Automatically coregistered images can be generated from the same sample. Imaging applications in ophthalmology, developmental biology, and plant science are demonstrated. For the first time, in a familiar microscopic fluorescence imaging setting, this trimodality microscope provides a platform for future biological and medical discoveries. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：6

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