CHARACTERIZATION OF DIFFERENTLY EXPRESSED GENES DURING THE INTERACTION BETWEEN 'FUJI' APPLES AND Botryosphaeria dothidea

被引：2
作者：
Corrent, Adriana Regina [1 ]
Schwarz, Ligia Loss [2 ]
Oster, Andreia Hansen
de Moraes, Marcelo Gravina [2 ]
Bender, Renar Joao [2 ]
机构：
[1] CEFET, Januaria, MG, Brazil
[2] Univ Fed Rio Grande do Sul, Fac Agron, Porto Alegre, RS, Brazil
来源：
REVISTA BRASILEIRA DE FRUTICULTURA | 2009年 / 31卷 / 01期
关键词：
Malus domestica; white-rot; reverse transcriptase; OXIDATIVE STRESS; RESISTANCE GENE; MALUS-DOMESTICA; PROTEINS; FRUIT;
D O I：
10.1590/S0100-29452009000100038
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The aim of this study was to investigate the interaction between the Botryosphaeria dothidea fungi and 'Fuji' apples using the Differential Display RT-PCR technique. The cDNA of infected and non infected fruits by the fungus was amplified using a combination of 15 primers. Four hundred fragments of differentially expressed cDNA were isolated, and 120 of them were sequenced and compared with available sequences at GenBank database, using BLASTX software. Obtained sequences were similar to methalothionines, an allergenic profiline, a resistance linked protein, and a phosphatase.
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收藏
页码：268 / 272
页数：5
相关论文
共 19 条
[1]   Role of superoxide dismutases (SODs) in controlling oxidative stress in plants [J].
Alscher, RG ;
Erturk, N ;
Heath, LS .
JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (372) :1331-1341
[2]   Application of differential display RT-PCR to the analysis of gene expression in a plant-fungus interaction [J].
Benito, EP ;
Prins, T ;
vanKan, JAL .
PLANT MOLECULAR BIOLOGY, 1996, 32 (05) :947-957
[3]   Characterisation of Mal d 1-related genes in Malus [J].
Beuning, LL ;
Bowen, JH ;
Persson, HA ;
Barraclough, D ;
Bulley, S ;
MacRae, EA .
PLANT MOLECULAR BIOLOGY, 2004, 55 (03) :369-388
[4]  
Boneti JIS, 1999, MANUAL IDENTIFICACAO
[5]   Expression of the Arabidopsis abi1-1 mutant allele inhibits proteinase inhibitor wound-induction in tomato [J].
Carrera, E ;
Prat, S .
PLANT JOURNAL, 1998, 15 (06) :765-771
[6]   Characterization by suppression subtractive hybridization of transcripts that are differentially expressed in leaves of apple scab-resistant and susceptible cultivars of Malus domestica [J].
Degenhardt, J ;
Al-Masri, AN ;
Kürkcüoglu, S ;
Szankowski, I ;
Gau, AE .
MOLECULAR GENETICS AND GENOMICS, 2005, 273 (04) :326-335
[7]   Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica) [J].
Gao, ZS ;
van de Weg, WE ;
Schaart, JG ;
van der Meer, IM ;
Kodde, L ;
Laimer, M ;
Breiteneder, H ;
Hoffmann-Sommergruber, K ;
Gilissen, LJWJ .
THEORETICAL AND APPLIED GENETICS, 2005, 110 (03) :479-491
[8]   FUNCTION AND AUTO-REGULATION OF YEAST COPPERTHIONEIN [J].
HAMER, DH ;
THIELE, DJ ;
LEMONTT, JE .
SCIENCE, 1985, 228 (4700) :685-690
[9]   DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].
LIANG, P ;
PARDEE, AB .
SCIENCE, 1992, 257 (5072) :967-971
[10]  
LIU JJ, 2004, MOL GENETICS GENOMIC, V270
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