High-resolution image reconstruction in fluorescence microscopy with patterned excitation

被引：69

作者：

Heintzmann, Rainer ^{[1
]}

Benedetti, Pier A.

机构：

[1] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England

[2] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

[3] CNR, Ist Proc Chim Fis, I-56100 Pisa, Italy

来源：

APPLIED OPTICS | 2006年 / 45卷 / 20期

关键词：

D O I：

10.1364/AO.45.005037

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We discuss data treatment strategies in structured illumination microscopy, using simulated and experimental data. In the setup, the illumination pattern is generated by projecting a movable pinhole mask into the sample, and a wide-field fluorescence microscope image is acquired for each pattern position. The structured illumination data obtained from a two-dimensional illumination pattern can be treated by projection strategies such as in video confocal microscopy (sum, maximum, maximum minus minimum, and superconfocal), by a scaled subtraction of the out-of-focus estimate, or by a modified version of the Fourier-space treatment, as is known for data from one-dimensional structured illumination. We investigate the influence of some data processing strategies on unwanted effects such as residual patterning and local deviations from linearity in the reconstructed intensity. (C) 2006 Optical Society of America.

引用

页码：5037 / 5045

页数：9

共 27 条

[11] Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples [J].

Fukano, T ;

Miyawaki, A .

APPLIED OPTICS, 2003, 42 (19) :4119-4124

[12] Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy [J].

Gustafsson, MGL .

JOURNAL OF MICROSCOPY, 2000, 198 (02) :82-87

[13] Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination [J].

Gustafsson, MGL ;

Agard, DA ;

Sedat, JW .

THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION PROCESSING VII, 2000, 3919 :141-150

[14]

GUSTAFSSON MGL, 1997, Patent No. 5671085

[15]

GUSTAFSSON MGL, 2001, COMMUNICATION

[16]

Hanley QS, 1999, J MICROSC-OXFORD, V196, P317, DOI 10.1046/j.1365-2818.1999.00602.x

[17] Saturated patterned excitation microscopy with two-dimensional excitation patterns [J].

Heintzmann, R .

MICRON, 2003, 34 (6-7) :283-291

[18] Laterally modulated excitation microscopy: Improvement of resolution by using a diffraction grating [J].

Heintzmann, R ;

Cremer, C .

OPTICAL BIOPSIES AND MICROSCOPIC TECHNIQUES III, PROCEEDINGS OF, 1999, 3568 :185-196

[19] Saturated patterned excitation microscopy - a concept for optical resolution improvement [J].

Heintzmann, R ;

Jovin, TM ;

Cremer, C .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2002, 19 (08) :1599-1609

[20] A dual path programmable array microscope (PAM): simultaneous acquisition of conjugate and non-conjugate images [J].

Heintzmann, R ;

Hanley, QS ;

Arndt-Jovin, D ;

Jovin, TM .

JOURNAL OF MICROSCOPY-OXFORD, 2001, 204 (02) :119-135

← 1 2 3 →