Confinement of single macromolecules in free solution using a hydrodynamic trap

被引：0
|
作者：
Tanyeri, Melikhan [1 ]
机构：
[1] Istanbul Sehir Univ, Dept Elect & Elect Engn, Uskudar Istanbul, Turkey
来源：
MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS XII | 2014年 / 8976卷
关键词：
Micro- and nanomanipulation; tweezers; trapping; hydrodynamic trap; microfluidics; BROWNIAN-MOTION; QUANTUM DOTS; MANIPULATION; CELLS; PARTICLES; MICROPARTICLES; NANOPARTICLES; BIOMOLECULES; OBJECTS;
D O I：
10.1117/12.2039063
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We demonstrate the feasibility of trapping and manipulating individual macromolecules such as globular proteins (similar to 5 nm in diameter) in free solution using a flow-based, microfluidic confinement method. This new method enables confinement of small nanoscale objects in free solution by utilizing a planar extensional flow created at a microchannel junction. The fluid flow based confinement method described in this work expands the micro/nanomanipulation toolbox by offering a powerful and versatile platform for non-perturbative observation and analysis of single macromolecules in free solution without force fields (electrical, magnetic, optical and acoustic) and surface immobilization.
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页数：6
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