Isolation and partial characterization of a neutral polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru and its antioxidant and immunomodulatory activities

被引：76

作者：

Li, Jing-En ^{[1
]}

Nie, Shao-Ping ^{[1
]}

Xie, Ming-Yong ^{[1
]}

Li, Chang ^{[1
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2014年 / 6卷

基金：

中国国家自然科学基金;

关键词：

Mosla chinensis Maxim. cv; Jiangxiangru; Polysaccharide; Partial characterization; Antioxidant; Immunomodulatory activities; ALDITOL ACETATE STANDARDS; DIFFERENT FRACTIONS; RAPID SYNTHESIS; GC-MS;

D O I：

10.1016/j.jff.2013.11.007

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A neutral polysaccharide MP-1 was isolated and purified from Mosla chinensis Maxim. cv. Jiangxiangru. The average molecular weight was 587.53 kDa and it was composed of glucose, galactose, arabinose, mannose and rhamnose in a molar ratio of 16.02:10.01:4.92:4.62:2.23, and trace of fucose. Partial characterization of MP-1 was elucidated by methylation analysis. MP-1 was composed of 1,4-Glcp (31.76%), followed by 1,2-Rhap (17.11%), 1,2,4-Rhap (6.47%), 1,3,4-Galp (5.05%), 1,4-Manp (3.61%), 1,2,4-Manp (2.55%), 1,5-Araf (3.10%) and 1,4,6-Glcp (1.53%). The branching points were located at O-2, O-4 or O-6 with alpha-Araf (4.01%), alpha-Rhap (4.31%), alpha-Fucp (2.39%), beta-Galp (11.02%) and beta-Glcp (4.04%) as terminal residues. MP-1 exhibited a dose-dependent enhancement in antioxidant assays in this study. Immunomodulatory effects of MP-1 were assessed by splenocyte proliferation test and nitric oxide production of mouse peritoneal macrophages. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：410 / 418

页数：9

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