Delavatine A, an unusual isoquinoline alkaloid exerts anti-inflammation on LPS-induced proinflammatory cytokines production by suppressing NF-κB activation in BV-2 microglia

被引：33

作者：

Xie, Qing ^{[1
]}

Wu, Guo-Zhen ^{[2
]}

Yang, Niao ^{[2
]}

Shen, Yun-Heng ^{[2
]}

Tang, Jian ^{[1
]}

Zhang, Wei-Dong ^{[2
]}

机构：

[1] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China

[2] Second Mil Med Univ, Sch Pharm, Dept Phytochem, Shanghai 200433, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 502卷 / 02期

关键词：

Delavatine A; Anti-inflammation; Cytokine; NO; NF-kappa B; Microglia; NITRIC-OXIDE SYNTHASE; PATHWAYS; DISEASES; CELLS; LIPOPOLYSACCHARIDE; TRANSCRIPTION; MACELIGNAN; EXPRESSION; MEDIATORS; BRAIN;

D O I：

10.1016/j.bbrc.2018.05.144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Delavatine A, an unusual isoquinoline alkaloid isolated from I. delavayi, was first studied for antiinflammatory effect using lipopolysaccharide (LPS)-induced BV-2 microglia. In the present study, we found that delavatine A substantially suppressed the LPS-induced pro-inflammatory mediators, nitric oxide (NO), and tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), interleukin-1 beta (1-1 beta) in BV-2 microglial cells. These effects resulted from the inhibition of their regulatory genes inducible NO synthase (iNOS), cycloxygenase-2 (COX-2) and TNF-alpha, IL-6, IL-beta. In addition, we examined several pathways related to inflammation. The results revealed that delavatine A significantly decreased LPS-induced the activation of nuclear factor-kappa B (NF-kappa B) by suppressing the p65 subunits, and the phosphorylation of IKBot, while not related to PI3K/Akt or MAPK pathways. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：202 / 208

页数：7

共 35 条

[1]

Ahmad N, 2002, J LEUKOCYTE BIOL, V71, P1005

[2] Anti-inflammatory Activity of a Honey Flavonoid Extract on Lipopolysaccharide-Activated N13 Microglial Cells [J].

Candiracci, Manila ;

Piatti, Elena ;

Dominguez-Barragan, Maria ;

Garcia-Antras, Daniel ;

Morgado, Bruno ;

Ruano, Diego ;

Gutierrez, Juan F. ;

Parrado, Juan ;

Castano, Angelica .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (50) :12304-12311

[3] Anti-inflammatory effects of spermidine in lipopolysaccharide-stimulated BV2 microglial cells [J].

Choi, Yung Hyun ;

Park, Hye Young .

JOURNAL OF BIOMEDICAL SCIENCE, 2012, 19

[4] Macelignan attenuates LPS-induced inflammation and reduces LPS-induced spatial learning impairments in rats [J].

Cui, Chun-Ai ;

Jin, Da-Qing ;

Hwang, Yoo Kyeong ;

Lee, Im-Soon ;

Hwang, Jae Kwan ;

Ha, Ilho ;

Han, Jung-Soo .

NEUROSCIENCE LETTERS, 2008, 448 (01) :110-114

[5] Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia [J].

de Oliveira, Antonio C. P. ;

Candelario-Jalil, Eduardo ;

Langbein, Julia ;

Wendeburg, Lena ;

Bhatia, Harsharan S. ;

Schlachetzki, Johannes C. M. ;

Biber, Knut ;

Fiebich, Bernd L. .

JOURNAL OF NEUROINFLAMMATION, 2012, 9

[6] Microglial activation and its implications in the brain diseases [J].

Dheen, S. Thameem ;

Kaur, Charanjit ;

Ling, Eng-Ang .

CURRENT MEDICINAL CHEMISTRY, 2007, 14 (11) :1189-1197

[7] NF-kB in development and progression of human cancer [J].

Dolcet, X ;

Llobet, D ;

Pallares, J ;

Matias-Guiu, X .

VIRCHOWS ARCHIV, 2005, 446 (05) :475-482

[8]

Gilmore T., 2006, ONCOGENE, V2006, P5

[9] NF-κB activation by reactive oxygen species:: Fifteen years later [J].

Gloire, Geoffrey ;

Legrand-Poels, Sylvie ;

Piette, Jacques .

BIOCHEMICAL PHARMACOLOGY, 2006, 72 (11) :1493-1505

[10] Microglia as mediators of inflammatory and degenerative diseases [J].

González-Scarano, F ;

Baltuch, G .

ANNUAL REVIEW OF NEUROSCIENCE, 1999, 22 :219-240

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