Recent applications of fluorescence correlation spectroscopy in live systems

被引：101
作者：
Machan, Radek
Wohland, Thorsten
机构：
[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117557, Singapore
[2] Natl Univ Singapore, Dept Chem, Singapore 117557, Singapore
[3] Natl Univ Singapore, Ctr BioImaging Sci, Singapore 117557, Singapore
来源：
FEBS LETTERS | 2014年 / 588卷 / 19期
关键词：
Fluorescence cross-correlation spectroscopy; Image correlation spectroscopy; Imaging FCS; Spatio-temporal image correlation spectroscopy; Fluorescence fluctuation spectroscopy; CROSS-CORRELATION SPECTROSCOPY; IMAGE CORRELATION SPECTROSCOPY; LIFETIME CORRELATION SPECTROSCOPY; INFLUENZA-A VIRUS; PHOTON-COUNTING HISTOGRAM; TOTAL INTERNAL-REFLECTION; LIVING CELLS; MEMBRANE DYNAMICS; PLASMA-MEMBRANE; DISSOCIATION-CONSTANT;
D O I：
10.1016/j.febslet.2014.03.056
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluorescence correlation spectroscopy (FCS) is a widely used technique in biophysics and has helped address many questions in the life sciences. It provides important advantages compared to other fluorescence and biophysical methods. Its single molecule sensitivity allows measuring proteins within biological samples at physiological concentrations without the need of overexpression. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions even in live organisms. And its reliance on simple fluorescence intensity and its fluctuations makes it widely applicable. In this review we focus on applications of FCS in live samples, with an emphasis on work in the last 5 years, in the hope to provide an overview of the present capabilities of FCS to address biologically relevant questions. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3571 / 3584
页数：14
相关论文
共 166 条
[1]   Genomic DNA Hypomethylation by Histone Deacetylase Inhibition Implicates DNMT1 Nuclear Dynamics [J].
Arzenani, Mohsen Karimi ;
Zade, Atosa Esteki ;
Ming, Yu ;
Vijverberg, Susanne J. H. ;
Zhang, Zhe ;
Khan, Zahidul ;
Sadique, Syed ;
Kallenbach, Lorenz ;
Hu, LiFu ;
Vukojevic, Vladana ;
Ekstrom, Tomas J. .
MOLECULAR AND CELLULAR BIOLOGY, 2011, 31 (19) :4119-4128
[2]   Replication-Competent Influenza A Virus That Encodes a Split-Green Fluorescent Protein-Tagged PB2 Polymerase Subunit Allows Live-Cell Imaging of the Virus Life Cycle [J].
Avilov, Sergiy V. ;
Moisy, Dorothee ;
Munier, Sandie ;
Schraidt, Oliver ;
Naffakh, Nadia ;
Cusack, Stephen .
JOURNAL OF VIROLOGY, 2012, 86 (03) :1433-1448
[3]   Temperature dependence of diffusion in model and live cell membranes characterized by imaging fluorescence correlation spectroscopy [J].
Bag, Nirmalya ;
Yap, Darilyn Hui Xin ;
Wohland, Thorsten .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2014, 1838 (03) :802-813
[4]   Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy [J].
Bag, Nirmalya ;
Ali, Ashraf ;
Chauhan, Virander Singh ;
Wohland, Thorsten ;
Mishra, Aseem .
CHEMICAL COMMUNICATIONS, 2013, 49 (80) :9155-9157
[5]   Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin [J].
Bancaud, Aurelien ;
Huet, Sebastien ;
Daigle, Nathalie ;
Mozziconacci, Julien ;
Beaudouin, Joel ;
Ellenberg, Jan .
EMBO JOURNAL, 2009, 28 (24) :3785-3798
[6]   VEGF autoregulates its proliferative and migratory ERK1/2 and p38 cascades by enhancing the expression of DUSP1 and DUSP5 phosphatases in endothelial cells [J].
Bellou, Sofia ;
Hink, Mark A. ;
Bagli, Eleni ;
Panopoulou, Ekaterini ;
Bastiaens, Philippe I. H. ;
Murphy, Carol ;
Fotsis, Theodore .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2009, 297 (06) :C1477-C1489
[7]   Mitochondrial dynamics in heart cells: Very low amplitude high frequency fluctuations in adult cardiomyocytes and flow motion in non beating Hl-1 cells [J].
Beraud, Nathalie ;
Pelloux, Sophie ;
Usson, Yves ;
Kuznetsov, Andrey V. ;
Ronot, Xavier ;
Tourneur, Yves ;
Saks, Valdur .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2009, 41 (02) :195-214
[8]   Spatio-Temporal Plasticity in Chromatin Organization in Mouse Cell Differentiation and during Drosophila Embryogenesis [J].
Bhattacharya, Dipanjan ;
Talwar, Shefali ;
Mazumder, Aprotim ;
Shivashankar, G. V. .
BIOPHYSICAL JOURNAL, 2009, 96 (09) :3832-3839
[9]   Cell Cycle-Dependent Binding Modes of the Ran Exchange Factor RCC1 to Chromatin [J].
Bierbaum, Martin ;
Bastiaens, Philippe I. H. .
BIOPHYSICAL JOURNAL, 2013, 104 (08) :1642-1651
[10]   Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets [J].
Bieschke, J ;
Giese, A ;
Schulz-Schaeffer, W ;
Zerr, I ;
Poser, S ;
Eigen, M ;
Kretzschmar, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (10) :5468-5473
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