3D bioprinted graphene oxide-incorporated matrix for promoting chondrogenic differentiation of human bone marrow mesenchymal stem cells

被引：149

作者：

Zhou, Xuan ^{[1
]}

Nowicki, Margaret ^{[1
]}

Cui, Haitao ^{[1
]}

Zhu, Wei ^{[1
]}

Fang, Xiuqi ^{[1
]}

Miao, Shida ^{[1
]}

Lee, Se Jun ^{[1
]}

Keidar, Michael ^{[1
,2
]}

Zhang, Lijie Grace ^{[1
,2
,3
]}

机构：

[1] George Washington Univ, Dept Mech & Aerosp Engn, Washington, DC 20052 USA

[2] George Washington Univ, Dept Biomed Engn, Washington, DC 20052 USA

[3] George Washington Univ, Dept Med, Washington, DC 20052 USA

来源：

CARBON | 2017年 / 116卷

基金：

美国国家科学基金会;

关键词：

ARTICULAR-CARTILAGE REPAIR; OSTEOGENIC DIFFERENTIATION; IN-VITRO; REGENERATION; SCAFFOLDS; DESIGN; NANOFIBERS; HYDROGEL; DELIVERY; CULTURE;

D O I：

10.1016/j.carbon.2017.02.049

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Articular cartilage repair and regeneration are a challenging problem worldwide due to the extremely weak inherent regenerative capacity of cartilaginous tissue. As an emerging tissue engineering scaffold fabrication technology, 3D bioprinting has shown great promise in fabricating customizable artificial tissue matrices with hierarchical structures. The goal of the present study is to investigate 3D bioprinted graphene oxide (GO)-doped gelatin based scaffolds for promoting chondrogenic differentiation of human bone marrow mesenchymal stem cells (MSCs). In the current study, GO-gelatin methacrylate (GeIMA) poly (ethylene glycol) diacrylate (PEGDA) was prepared as a biocompatible photopolymerizable bioink. GO, a multifunctional carbon based nanomaterial, was incorporated into the bioink for promoting chondrogenic differentiation. Finally, the 3D printed GeIMA-PEGDA-GO scaffold with hierarchical structures was fabricated via our novel table-top stereolithography-based printer. Results showed that GeIMA-PEGDA-GO scaffolds greatly promoted the glycosaminoglycan, and collagen levels after GO induced chondrogenic differentiation of hMSCs. Moreover, the Collagen II, SOX 9, and Aggrecan gene expressions associated with chondrogenesis were greatly promoted on the scaffolds. This study demonstrated that customizable 3D printed GeIMA-PEGDA-GO scaffolds are excellent candidates for promoting chondrogenic differentiation of hMSCs and are therefore promising candidates for future cartilage regenerative medicine applications. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：615 / 624

页数：10

共 44 条

[1] Controlling Cell Behavior Through the Design of Polymer Surfaces [J].

Alves, Natalia M. ;

Pashkuleva, Iva ;

Reis, Rui L. ;

Mano, Joao F. .

SMALL, 2010, 6 (20) :2208-2220

[2] Age related changes in human articular chondrocyte yield, proliferation and post-expansion chondrogenic capacity [J].

Barbero, A ;

Grogan, S ;

Schäfer, D ;

Heberer, M ;

Mainil-Varlet, P ;

Martin, I .

OSTEOARTHRITIS AND CARTILAGE, 2004, 12 (06) :476-484

[3]

Becerra J, 2010, TISSUE ENG PART B-RE, V16, P617, DOI 10.1089/ten.TEB.2010.0191

[4] Sox9 is required for cartilage formation [J].

Bi, WM ;

Deng, JM ;

Zhang, ZP ;

Behringer, RR ;

de Crombrugghe, B .

NATURE GENETICS, 1999, 22 (01) :85-89

[5] Noncovalent functionalization of graphene with end-functional polymers [J].

Choi, Eun-Young ;

Han, Tae Hee ;

Hong, Jihyun ;

Kim, Ji Eun ;

Lee, Sun Hwa ;

Kim, Hyun Wook ;

Kim, Sang Ouk .

JOURNAL OF MATERIALS CHEMISTRY, 2010, 20 (10) :1907-1912

[6] The in vitro and in vivo toxicity of graphene quantum dots [J].

Chong, Yu ;

Ma, Yufei ;

Shen, He ;

Tu, Xiaolong ;

Zhou, Xuan ;

Xu, Jiaying ;

Dai, Jianwu ;

Fan, Saijun ;

Zhang, Zhijun .

BIOMATERIALS, 2014, 35 (19) :5041-5048

[7] Bioinspired nanofibers support chondrogenesis for articular cartilage repair [J].

Coburn, Jeannine M. ;

Gibson, Matthew ;

Monagle, Sean ;

Patterson, Zachary ;

Elisseeff, Jennifer H. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (25) :10012-10017

[8] The influence of structural design of PLGA/collagen hybrid scaffolds in cartilage tissue engineering [J].

Dai, Wenda ;

Kawazoe, Naoki ;

Lin, Xiaoting ;

Dong, Jian ;

Chen, Guoping .

BIOMATERIALS, 2010, 31 (08) :2141-2152

[9]

Delorme Bruno, 2007, V140, P67

[10] Determining synthesis region of the single wall carbon nanotubes in arc plasma volume [J].

Fang, Xiuqi ;

Shashurin, Alexey ;

Teel, George ;

Keidar, Michael .

CARBON, 2016, 107 :273-280

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