VOLATILE COMPOUNDS OF Nectandra salicina (LAURACEAE) FROM COSTA RICA AND THEIR CYTOTOXIC ACTIVITY ON CELL LINES

The chemical composition of the volatiles of Nectandra salicina growing wild in Costa Rica was determined by capillary GC/FID and GC/ MS. Thirty-seven and forty-two compounds were identified in the leaf and branch oils respectively corresponding to about 92.6 and 86.2% of the total amount of the oils. The major components of the leaf oil were: atractylone (14.6%), viridiflorene (10.1%), alpha-pinene (9.4%), beta-caryophyllene (7.2%), alpha-humulene (7.0%), delta-cadinene (6.1%), beta-pinene (6.0%) and germacrene D (5.8%). The major components of the branch oil were: atractylone (21.1%), germacrene D (10.7%), viridiflorene (7.9%) and 7-epi-alpha-selinene (5.0%). When the oils were tested on different cell lines, all the LD50 values were higher than 150 mu g/mL, with values very similar for the leaf and branch oils. Low toxicity could be explained by antagonistic effects among the main compounds present in the oils.