Study of Circulating MicroRNA-125b Levels in Serum Exosomes in Advanced Melanoma

被引:119
作者
Alegre, Estibaliz [1 ]
Sanmamed, Miguel F. [2 ]
Rodriguez, Carmen [1 ]
Carranza, Omar [2 ]
Martin-Algarra, Salvador [2 ]
Gonzalez, Alvaro [1 ]
机构
[1] Univ Navarra Clin, Biochem Lab, Pamplona 31008, Spain
[2] Univ Navarra Clin, Dept Med Oncol, Pamplona 31008, Spain
关键词
EXPRESSION PROFILES; MALIGNANT-MELANOMA; PLASMA; CELLS; MIRNAS; CANCER; PROGRESSION; BIOMARKERS; DIAGNOSIS; MIR-125B;
D O I
10.5858/arpa.2013-0134-OA
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Context.-Malignant melanoma is an aggressive tumor that produces exosomes, which contain microRNAs (miRNAs) that could be of utility in following tumoral cell dysregulation. MicroR-125b is a miRNA whose down-regulation seems to be implicated in melanoma progression. Objective.-To analyze miR-125b levels in serum, and in exosomes obtained from serum, from patients with advanced melanoma. Design.-Serum samples were obtained from 21 patients with advanced melanoma, from 16 disease-free patients with melanoma, and from 19 healthy volunteers. Exosomes were isolated from serum by precipitation, and miR-16 and miR-125b levels were quantified by real-time polymerase chain reaction. Results.-MicroR-16, but not miR-125b, was detected in all samples, and miR-16 levels were significantly higher in serum than they were in exosomes. MicroR-16 expression levels did not differ significantly between the 2 groups (patients with melanoma and healthy donors). There was a significant relationship between miR-125b and miR-16 levels in exosomes. Additionally, miR-125b levels in exosomes were significantly lower in patients with melanoma compared with disease-free patients with melanoma and healthy controls. Conclusions.-Exosomes can provide a suitable material to measure circulating miRNA in melanoma, and miR-16 can be used as an endogenous normalizer. Lower levels of miR-125b in exosomes obtained from serum are associated with advanced melanoma disease, probably reflecting the tumoral cell dysregulation.
引用
收藏
页码:828 / 832
页数:5
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