Comparison of two commercial carbapenemase gene confirmatory assays in multiresistant Enterobacteriaceae and Acinetobacter baumannii-complex

被引：5

作者：

Roesner, Stephan ^{[1
]}

Gehlweiler, Kevin ^{[1
]}

Kuesters, Uta ^{[1
]}

Kolbert, Mathias ^{[1
]}

Huebner, Kirsten ^{[1
]}

Pfennigwerth, Niels ^{[2
]}

Mack, Dietrich ^{[1
]}

机构：

[1] Bioscientia Inst Med Diagnost, Microbiol Infect Dis, Ingelheim, Germany

[2] Ruhr Univ Bochum, Dept Med Microbiol, Bochum, Germany

来源：

PLOS ONE | 2018年 / 13卷 / 05期

关键词：

GRAM-NEGATIVE BACILLI; CHECK-DIRECT CPE; ESCHERICHIA-COLI; RECTAL SWABS; SPREAD; PERFORMANCE; BLA(OXA-23); PCR;

D O I：

10.1371/journal.pone.0197839

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Multidrug-resistant Gram-negative bacilli (MDR-GNB) producing carbapenemases are increasing at an alarming speed. Rapid confirmation of carbapenemase type will be an important diagnostic step in clinical microbiology laboratories not only to reduce the risk of transmissions but also for optimising antibiotic therapy in the future. We compared diagnostic reliability of two commercially available molecular assays (Check-Direct CPE vs. AID line probe assay) for detection and typing of carbapenemase genes in 80 well-characterized isolates of MDR-GNB. Respective strains were isolated in various clinical specimens at our clinical microbiology laboratory. The reference standard included confirmation of carbapenemase-production at the molecular level at the German National Reference Laboratory for Multidrug-resistant Gram-negative bacteria (Ruhr-University Bochum, Germany). 53 Enterobacteriaceae and 27 members of the A. baumannii-complex were used in this study. The tested assays appeared highly reliable to confirm carbapenemase-producing Enterobacteriaceae (CPE) with respective sensitivities of 97.7%, but are currently unsuitable for analysis of members of the A. baumannii-complex. Both assays are easy to perform and rapid tools for confirmation and typing of the most common carbapenemase genes in Enterobacteriaceae. Implementation should be possible for any clinical microbiology laboratory with Check-Direct CPE being easier to handle and having less technological requirements.

引用

页数：7

共 16 条

[1] Carbapenemase-producing Enterobacteriaceae in Europe: assessment by national experts from 38 countries, May 2015 [J].

Albiger, B. ;

Glasner, C. ;

Struelens, M. J. ;

Grundmann, H. ;

Monnet, D. L. .

EUROSURVEILLANCE, 2015, 20 (45) :17-34

[2]

[Anonymous], LANCET INFECT DIS, DOI DOI 10.1016/S1473-3099(16)30257-2

[3] Performance of the BD MAX™ instrument with Check-Direct CPE real-time PCR for the detection of carbapenemase genes from rectal swabs, in a setting with endemic dissemination of carbapenemase-producing Enterobacteriaceae [J].

Antonelli, Alberto ;

Arena, Fabio ;

Giani, Tommaso ;

Colavecchio, Olga Lorenza ;

Valeva, Stoyanka Valentinova ;

Paule, Suzanne ;

Boleij, Peter ;

Rossolini, Gian Maria .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2016, 86 (01) :30-34

[4] Evaluation of the AID carbapenemase line probe assay for rapid detection and identification of carbapenemase genes in Gram-negative bacilli [J].

Bloemberg, Guido V. ;

Braun-Kiewnick, Andrea ;

Tedrup, Jan ;

Meijerink, Carla ;

Durer, Elena ;

Ritter, Claudia ;

Keller, Peter M. ;

Hombach, Michael .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2017, 72 (07) :1948-1954

[5] Rapid evolution and spread of carbapenemases among Enterobacteriaceae in Europe [J].

Canton, R. ;

Akova, M. ;

Carmeli, Y. ;

Giske, C. G. ;

Glupczynski, Y. ;

Gniadkowski, M. ;

Livermore, D. M. ;

Miriagou, V. ;

Naas, T. ;

Rossolini, G. M. ;

Samuelsen, O. ;

Seifert, H. ;

Woodford, N. ;

Nordmann, P. .

CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (05) :413-431

[6] Spotlight on ceftazidime/avibactam: a new option for MDR Gram-negative infections [J].

Falcone, Marco ;

Paterson, David .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2016, 71 (10) :2713-2722

[7] Multicentre evaluation of the Check-Direct CPE® assay for direct screening of carbapenemase-producing Enterobacteriaceae from rectal swabs [J].

Huang, Te-Din ;

Bogaerts, Pierre ;

Ghilani, Enes ;

Heinrichs, Amelie ;

Gavage, Pierre ;

Roisin, Sandrine ;

Willems, Elise ;

Verbruggen, Anne-Marie ;

Francart, Hugo ;

Denis, Olivier ;

Senterre, Jean-Marc ;

Glupczynski, Youri .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2015, 70 (06) :1669-1673

[8] Unusual Detection of an Acinetobacter Class D Carbapenemase Gene, blaOXA-23, in a Clinical Escherichia coli Isolate [J].

La, My-Van ;

Jureen, Roland ;

Lin, Raymond T. P. ;

Teo, Jeanette W. P. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2014, 52 (10) :3822-3823

[9] Clinical Performance of Check-Direct CPE, a Multiplex PCR for Direct Detection of blaKPC, blaNDM and/or blaVIM, and blaOXA-48 from Perirectal Swabs [J].

Lau, Anna F. ;

Fahle, Gary A. ;

Kemp, Margaret A. ;

Jassem, Agatha N. ;

Dekker, John P. ;

Frank, Karen M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (12) :3729-3737

[10] Recent advances in the laboratory detection of carbapenemase-producing Enterobacteriaceae [J].

Matsumura, Yasufumi ;

Pitout, Johann D. .

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2016, 16 (07) :783-794

← 1 2 →