Colwellia meonggei sp nov., a novel gammaproteobacterium isolated from sea squirt Halocynthia roretzi

被引:10
作者
Kim, Young-Ok [1 ]
Park, Sooyeon [2 ]
Nam, Bo-Hye [1 ]
Jung, Yong-Taek [2 ]
Kim, Dong-Gyun [1 ]
Yoon, Jung-Hoon [2 ]
机构
[1] Natl Fisheries Res & Dev Inst, Biotechnol Res Div, Pusan 619705, South Korea
[2] Sungkyunkwan Univ, Dept Food Sci & Biotechnol, Suwon, South Korea
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2013年 / 104卷 / 06期
关键词
Colwellia meonggei; Novel species; Polyphasic taxonomy; Sea squirt (Halocynthia roretzi); TIDAL FLAT SEDIMENT; BACTERIAL SYSTEMATICS; EMENDED DESCRIPTION; THALASSOMONAS; GENUS; IDENTIFICATION; STRAINS;
D O I
10.1007/s10482-013-0022-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A Gram-negative, non-spore-forming, aerobic, motile and rod-shaped or ovoid bacterial strain, designated MA1-3(T), was isolated from a sea squirt (Halocynthia roretzi) collected from the South sea in South Korea. Strain MA1-3(T) was found to grow optimally at 25 A degrees C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain MA1-3(T) fell within the clade comprising Colwellia species, clustering coherently with the type strains of Colwellia aestuarii, Colwellia polaris and Colwellia chukchiensis, showing sequence similarity values of 97.2, 96.4 and 95.6 %, respectively. It exhibited 16S rRNA gene sequence similarity values of 93.9-96.1 % to the type strains of the other Colwellia species. Strain MA1-3(T) was found to contain Q-8 as the predominant ubiquinone and C-16:1 omega 7c and/or C-16:1 omega 6c, C-16:0 and C-16:1 omega 9c as the major fatty acids. The DNA G+C content of strain MA1-3(T) was determined to be 39.1 mol% and its mean DNA-DNA relatedness value with the type strain of C. aestuarii was 13 +/- A 5.4 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separated from other Colwellia species. On the basis of the data presented, strain MA1-3(T) is considered to represent a novel species of the genus Colwellia, for which the name Colwellia meonggei sp. nov. is proposed. The type strain is MA1-3(T) (=KCTC 32380(T) = CECT 8302(T)).
引用
收藏
页码:1021 / 1027
页数:7
相关论文
共 33 条
[1]  
[Anonymous], 1990, Technical Note 101
[2]  
Barrow GI., 1993, COWAN STEELS MANUAL, V3th
[3]  
Baumann P., 1981, The prokaryotes, P1302
[4]   Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.:: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22:6ω3) [J].
Bowman, JP ;
Gosink, JJ ;
McCammon, SA ;
Lewis, TE ;
Nichols, DS ;
Nichols, PD ;
Skerratt, JH ;
Staley, JT ;
McMeekin, TA .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :1171-1180
[5]   Muricauda ruestringensis gen. nov., sp nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment [J].
Bruns, A ;
Rohde, M ;
Berthe-Corti, L .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1997-2006
[6]   Colwellia asteriadis sp nov., a marine bacterium isolated from the starfish Asterias amurensis [J].
Choi, Fun Ju ;
Kwon, Hak Cheol ;
Koh, Hye Yeon ;
Kim, Young Sug ;
Yang, Hyun Ok .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2010, 60 :1952-1957
[7]   KINETIC STUDIES OF PIGMENT SYNTHESIS BY NON-SULFUR PURPLE BACTERIA [J].
COHENBAZIRE, G ;
SISTROM, WR ;
STANIER, RY .
JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1957, 49 (01) :25-68
[8]   ISOLATION OF AN OBLIGATELY BAROPHILIC BACTERIUM AND DESCRIPTION OF A NEW GENUS, COLWELLIA GEN-NOV [J].
DEMING, JW ;
SOMERS, LK ;
STRAUBE, WL ;
SWARTZ, DG ;
MACDONELL, MT .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1988, 10 (02) :152-160
[9]   FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS [J].
EZAKI, T ;
HASHIMOTO, Y ;
YABUUCHI, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :224-229
[10]   Thalassomonas actiniarum sp nov and Thalassomonas haliotis sp nov., isolated from marine animals [J].
Hosoya, Shoichi ;
Adachi, Kyoko ;
Kasai, Hiroaki .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009, 59 :686-690