Isolation and characterization of an AP2/ERF-type drought stress inducible transcription factor encoding gene from rice

被引：18

作者：

Mawlong, Ibandalin ^{[1
]}

Ali, Kishwar ^{[1
]}

Kurup, Devika ^{[1
]}

Yadav, Sangita

Tyagi, Aruna ^{[1
]}

机构：

[1] Indian Agr Res Inst, Div Biochem, New Delhi 110012, India

来源：

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 2014年 / 23卷 / 01期

关键词：

Rice; Transcription factor; AP2/EREBP; SHN; Drought; EPICUTICULAR WAX CONTENT; ARABIDOPSIS; TOLERANCE; SALT; DOMAIN; WATER; EFFICIENCY; FAMILY; ERF;

D O I：

10.1007/s13562-012-0185-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genomic DNA fragment of 964 bp corresponding to AP2/ERF family transcription factor was isolated from drought tolerant Oryza sativa L. cv. 'N-22' (AP2/ERF-'N-22') and Oryza sativa L. cv. 'Teipei 309' (AP2/ERF-jap). The cDNA sequence of AP2/ERF-'N-22' (732 bp) was amplified using gene specific primers. The gene contains two exons and a single intron. The deduced protein of the AP2/ERF-'N-22' contains a potential nuclear localization signal, a possible regulation domain and an AP2 DNA binding domain of 60 amino acids. Northern and RT-PCR analysis showed that transcript of AP2/ERF-'N-22' accumulates in response to WDS and the Southern analysis indicated the presence of single copy of AP2/ERF-'N-22' gene in Oryza sativa genome. Phylogenetic analysis of AP2/ERF family revealed that AP2/ERF-'N-22' belongs to group Va along with SHN clade of AP2/ERF protein which activates wax biosynthesis. A higher epicuticular wax content was observed at decreased RWC and positively correlated with AP2/ERF-'N-22' expression.

引用

页码：42 / 51

页数：10

共 37 条

[1] Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3 [J].

Abdeen, Ashraf ;

Schnell, Jaimie ;

Miki, Brian .

BMC GENOMICS, 2010, 11

[2] The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis [J].

Aharoni, A ;

Dixit, S ;

Jetter, R ;

Thoenes, E ;

van Arkel, G ;

Pereira, A .

PLANT CELL, 2004, 16 (09) :2463-2480

[3]

Alcamo J, 2000, 2 U KASS CTR ENV RES

[4] Differential antioxidative responses in transgenic peanut bear no relationship to their superior transpiration efficiency under drought stress [J].

Bhatnagar-Mathur, Pooja ;

Devi, M. Jyostna ;

Vadez, Vincent ;

Sharma, Kiran K. .

JOURNAL OF PLANT PHYSIOLOGY, 2009, 166 (11) :1207-1217

[5] PLANT PRODUCTIVITY AND ENVIRONMENT [J].

BOYER, JS .

SCIENCE, 1982, 218 (4571) :443-448

[6] WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis [J].

Broun, P ;

Poindexter, P ;

Osborne, E ;

Jiang, CZ ;

Riechmann, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (13) :4706-4711

[7] GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants [J].

Chen, Ming ;

Wang, Qiao-Yan ;

Cheng, Xian-Guo ;

Xu, Zhao-Shi ;

Li, an-Cheng Li ;

Ye, Xing-Guo ;

Xia, Lan-Qin ;

Ma, You-Zhi .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 353 (02) :299-305

[8] RAPID COLORIMETRIC METHOD FOR EPICUTICULAR WAX CONTENT OF SORGHUM LEAVES [J].

EBERCON, A ;

BLUM, A ;

JORDAN, WR .

CROP SCIENCE, 1977, 17 (01) :179-180

[9]

FAO, 2010, START LATEST BIENN M

[10] Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression [J].

Fujimoto, SY ;

Ohta, M ;

Usui, A ;

Shinshi, H ;

Ohme-Takagi, M .

PLANT CELL, 2000, 12 (03) :393-404

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