Synthesis, characterization, and DNA binding of two copper (II) complexes as DNA fluorescent probes

Two copper(II) complexes, [Cu(DAPT)(2)Cl]Cl center dot H2O and [Cu(DBM)(DAPT)Cl] [DAPT = 2,4-diamine- 6-(pyrazin-2-yl)-1,3,5-triazine], were synthesized and characterized. The complexes interact with ct-DNA through classical intercalation. Fluorescence intensity changes of 1 and 2 in the absence and presence of ct-DNA have been investigated for quantitative determination of ct-DNA with the limit of detection of 3.8 and 7.7 ng mL(-1). [Cu(DAPT)(2)Cl]Cl center dot H2O and [Cu(DBM)(DAPT)Cl] [DAPT = 2,4-diamine-6-(pyrazin-2-yl)-1,3,5-triazine] were synthesized and characterized by IR and UV spectroscopy, elemental analysis, TG-DTA, molar conductivity, and LC-MS. The interaction with calf thymus DNA (ct-DNA) of the two complexes has been studied using UV spectra, fluorescent spectra, cyclic voltammetry, and viscosity measurements. The complexes interact with ct-DNA through classical intercalation. Fluorescence intensity changes of 1 and 2 in the absence and presence of ct-DNA have been investigated for quantitative determination of ct-DNA with the limit of detection of 3.8 and 7.7 ng mL(-1), respectively. From the result, the two complexes are potentially sensitive DNA fluorescent probes.