Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase

被引：197

作者：

Cogoni, C ^{[1
]}

Macino, G ^{[1
]}

机构：

[1] Univ Rome La Sapienza, Sez Genet Mol, Dipartimento Biotecnol Cellulari & Ematol, I-00161 Rome, Italy

来源：

SCIENCE | 1999年 / 286卷 / 5448期

关键词：

D O I：

10.1126/science.286.5448.2342

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The phenomenon of posttranscriptional gene silencing (PTGS), which occurs when a transgene is introduced into a cell, is poorly understood. Here, the qde-3 gene, which is required for the activation and maintenance of gene silencing in the fungus Neurospora crassa, was isolated. Sequence analysis revealed that the qde-3 gene belongs to the RecQ DNA helicase family. The QDE3 protein may function in the DNA-DNA interaction between introduced transgenes or with an endogenous gene required for gene-silencing activation. In animals, genes that are homologous to RecQ protein, such as the human genes for Bloom's syndrome and Werner's syndrome, may also function in PTGS.

引用

页码：2342 / 2344

页数：3

共 25 条

[11] MUTAGENESIS AT THE AD-3A AND AD-3B LOCI IN HAPLOID UV-SENSITIVE STRAINS OF NEUROSPORA-CRASSA .4. COMPARISON OF DOSE-RESPONSE CURVES FOR MNNG, 4NQO AND ICR-170 INDUCED INACTIVATION AND MUTATION-INDUCTION [J].