Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase

被引：197
作者：
Cogoni, C [1 ]
Macino, G [1 ]
机构：
[1] Univ Rome La Sapienza, Sez Genet Mol, Dipartimento Biotecnol Cellulari & Ematol, I-00161 Rome, Italy
来源：
SCIENCE | 1999年 / 286卷 / 5448期
关键词：
D O I：
10.1126/science.286.5448.2342
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The phenomenon of posttranscriptional gene silencing (PTGS), which occurs when a transgene is introduced into a cell, is poorly understood. Here, the qde-3 gene, which is required for the activation and maintenance of gene silencing in the fungus Neurospora crassa, was isolated. Sequence analysis revealed that the qde-3 gene belongs to the RecQ DNA helicase family. The QDE3 protein may function in the DNA-DNA interaction between introduced transgenes or with an endogenous gene required for gene-silencing activation. In animals, genes that are homologous to RecQ protein, such as the human genes for Bloom's syndrome and Werner's syndrome, may also function in PTGS.
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页码：2342 / 2344
页数：3
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