Chemiluminescence method for the determination of glutathione in human serum using the Ru( phen)32+ -: KMnO4 system

被引：24
作者：
Han, He-You [1 ]
He, Zhi-Ke
Zeng, Yun-E
机构：
[1] Huazhong Normal Univ, Coll Sci, Dept Chem, Wuhan 430070, Peoples R China
[2] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
来源：
MICROCHIMICA ACTA | 2006年 / 155卷 / 3-4期
关键词：
chemiluminescence; Ru(phen)(3)(2+); glutathione; potassium permanganate;
D O I：
10.1007/s00604-006-0513-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple, rapid and specific chemiluminescence (CL) method for the determination of glutathione has been developed. The method is based on the enhanced CL of the reaction between Ru(phen)(3)(2+) (phen = 1,10-phenanthroline) and KMnO4 by glutathione in HCl medium. Under the optimum conditions, the response is linearly proportional to the concentration of glutathione between 1.5 x 10(-7) and 1.0 x 10(-5) mol L-1. The dection limit for glutathione (5.8 x 10(-8) mol L-1) is about 10 and 200 times better than those of the spectrophotometric method using Ellman regent and the Lucigenin - CL method, respectively. The final procedure allows the determination of glutathione in human serum with recoveries of 92%-108%. A satisfactory agreement was obtained with a mean relative difference of 2.5% compared to the HPLC method.
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收藏
页码：431 / 434
页数：4
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