A single dose of recombinant VSV-G-spike vaccine provides protection against SARS-CoV-2 challenge

被引：164

作者：

Yahalom-Ronen, Yfat ^{[1
]}

Tamir, Hadas ^{[1
]}

Melamed, Sharon ^{[1
]}

Politi, Boaz ^{[1
]}

Shifman, Ohad ^{[1
]}

Achdout, Hagit ^{[1
]}

Vitner, Einat B. ^{[1
]}

Israeli, Ofir ^{[1
]}

Milrot, Elad ^{[1
]}

Stein, Dana ^{[1
]}

Cohen-Gihon, Inbar ^{[1
]}

Lazar, Shlomi ^{[1
]}

Gutman, Hila ^{[1
]}

Glinert, Itai ^{[1
]}

Cherry, Lilach ^{[1
]}

Vagima, Yaron ^{[1
]}

Lazar, Shirley ^{[1
]}

Weiss, Shay ^{[1
]}

Ben-Shmuel, Amir ^{[1
]}

Avraham, Roy ^{[1
]}

Puni, Reut ^{[1
]}

Lupu, Edith ^{[1
]}

Bar-David, Elad ^{[1
]}

Sittner, Assa ^{[1
]}

Erez, Noam ^{[1
]}

Zichel, Ran ^{[1
]}

Mamroud, Emanuelle ^{[1
]}

Mazor, Ohad ^{[1
]}

Levy, Haim ^{[1
]}

Laskar, Orly ^{[1
]}

Yitzhaki, Shmuel ^{[1
]}

Shapira, Shmuel C. ^{[1
]}

Zvi, Anat ^{[1
]}

Beth-Din, Adi ^{[1
]}

Paran, Nir ^{[1
]}

Israely, Tomer ^{[1
]}

机构：

[1] Israel Inst Biol Res, Ness Ziona, Israel

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; INFECTION; PROTEIN;

D O I：

10.1038/s41467-020-20228-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of an efficient and cost-effective vaccine, suitable for mass immunization. Here, we show the development of a replication competent recombinant VSV-G-spike vaccine, in which the glycoprotein of VSV is replaced by the spike protein of SARS-CoV-2. In-vitro characterization of this vaccine indicates the expression and presentation of the spike protein on the viral membrane with antigenic similarity to SARS-CoV-2. A golden Syrian hamster in-vivo model for COVID-19 is implemented. We show that a single-dose vaccination results in a rapid and potent induction of SARS-CoV-2 neutralizing antibodies. Importantly, vaccination protects hamsters against SARS-CoV-2 challenge, as demonstrated by the abrogation of body weight loss, and alleviation of the extensive tissue damage and viral loads in lungs and nasal turbinates. Taken together, we suggest the recombinant VSV-G-spike as a safe, efficacious and protective vaccine against SARS-CoV-2. Here, the authors generate a replication-competent VSV based vaccine expressing SARS-CoV-2 spike protein and show protection in the hamster model with one dose. Analysis of the antibody response in mice shows induction of neutralizing antibodies and suggests a desirable Th1-biased response to the vaccine.

引用

页数：13

共 38 条

[1] SARS-CoV-2 Vaccines: Status Report [J].

Amanat, Fatima ;

Krammer, Florian .

IMMUNITY, 2020, 52 (04) :583-589

[2]

Bittencourt S. A, 2010, FASTQC QUALITY CONTR

[3] Coronavirus vaccine-associated lung immunopathology-what is the significance? [J].

Bottazzi, Maria Elena ;

Strych, Ulrich ;

Hotez, Peter J. ;

Corry, David B. .

MICROBES AND INFECTION, 2020, 22 (09) :403-404

[4] Replication-Competent Vesicular Stomatitis Virus Vaccine Vector Protects against SARS-CoV-2-Mediated Pathogenesis in Mice [J].

Case, James Brett ;

Rothlauf, Paul W. ;

Chen, Rita E. ;

Kafai, Natasha M. ;

Fox, Julie M. ;

Smith, Brittany K. ;

Shrihari, Swathi ;

McCune, Broc T. ;

Harvey, Ian B. ;

Keeler, Shamus P. ;

Bloyet, Louis-Marie ;

Zhao, Haiyan ;

Ma, Meisheng ;

Adams, Lucas J. ;

Winkler, Emma S. ;

Holtzman, Michael J. ;

Fremont, Daved H. ;

Whelan, Sean P. J. ;

Diamond, Michael S. .

CELL HOST & MICROBE, 2020, 28 (03) :465-+

[5]

Case James Brett, 2020, bioRxiv, DOI [10.1016/j.chom.2020.06.021, 10.1101/2020.05.18.102038]

[6] Simulation of the Clinical and Pathological Manifestations of Coronavirus Disease 2019 (COVID-19) in a Golden Syrian Hamster Model: Implications for Disease Pathogenesis and Transmissibility [J].

Chan, Jasper Fuk-Woo ;

Zhang, Anna Jinxia ;

Yuan, Shuofeng ;

Poon, Vincent Kwok-Man ;

Chan, Chris Chung-Sing ;

Lee, Andrew Chak-Yiu ;

Chan, Wan-Mui ;

Fan, Zhimeng ;

Tsoi, Hoi-Wah ;

Wen, Lei ;

Liang, Ronghui ;

Cao, Jianli ;

Chen, Yanxia ;

Tang, Kaiming ;

Luo, Cuiting ;

Cai, Jian-Piao ;

Kok, Kin-Hang ;

Chu, Hin ;

Chan, Kwok-Hung ;

Sridhar, Siddharth ;

Chen, Zhiwei ;

Chen, Honglin ;

To, Kelvin Kai-Wang ;

Yuen, Kwok-Yung .

CLINICAL INFECTIOUS DISEASES, 2020, 71 (09) :2428-2446

[7]

Dieterle M Eugenia, 2020, bioRxiv, DOI [10.1016/j.chom.2020.06.020, 10.1101/2020.05.20.105247]

[8] Recombinant vesicular stomatitis virus vector vaccines for WHO blueprint priority pathogens [J].

Fathi, Anahita ;

Dahlke, Christine ;

Addo, Marylyn M. .

HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2019, 15 (10) :2269-2285

[9] Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein [J].

Fukushi, S ;

Mizutani, T ;

Saijo, M ;

Matsuyama, S ;

Miyajima, N ;

Taguchi, F ;

Itamura, S ;

Kurane, I ;

Morikawa, S .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :2269-2274

[10] The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2 [J].

Gorbalenya, Alexander E. ;

Baker, Susan C. ;

Baric, Ralph S. ;

de Groot, Raoul J. ;

Drosten, Christian ;

Gulyaeva, Anastasia A. ;

Haagmans, Bart L. ;

Lauber, Chris ;

Leontovich, Andrey M. ;

Neuman, Benjamin W. ;

Penzar, Dmitry ;

Perlman, Stanley ;

Poon, Leo L. M. ;

Samborskiy, Dmitry V. ;

Sidorov, Igor A. ;

Sola, Isabel ;

Ziebuhr, John .

NATURE MICROBIOLOGY, 2020, 5 (04) :536-544

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