Chlamydia pneumoniae disrupts lipid metabolism in human umbilical vein endothelial cells

被引：11
作者：
Sun, Shan [1 ]
Cheng, Bei [1 ]
Wu, Xiaohua [1 ]
Wu, Qinqin [1 ]
Qi, Benling [1 ]
Wu, Jianping [2 ]
He, Ping [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Gerontol, Wuhan 430022, Hubei, Peoples R China
[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Cardiol, Wuhan 430022, Hubei, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2014年 / 10卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Chlamydia pneumoniae; endothelial cells; atherosclerosis; lipid metabolism; LOW-DENSITY-LIPOPROTEIN; OXIDIZED LDL; ADHESION MOLECULES; ACYL-COENZYME; CHOLESTEROL ACYLTRANSFERASE; ATHEROSCLEROSIS; INFECTION; ATHEROGENESIS; MACROPHAGES; EXPRESSION;
D O I：
10.3892/mmr.2014.2295
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Atherosclerosis is well established as a chronic inflammatory disorder, and Chlamydia pneumoniae is considered to be a risk factor for atherosclerotic development. Endothelial dysfunction, caused by oxidized low-density lipoprotein (ox-LDL) is an early atherosclerotic marker. However, the effect of C. pneumoniae on lipid metabolism in vascular endothelial cells is yet to be elucidated. The aim of the present study was to investigate the effects of C. pneumoniae on lipid metabolism in human umbilical vein endothelial cells (HUVECs). In the present study, LDL oxidation was found to be significantly induced in the supernatant, but not the cell lysates, of C. pneumoniae-infected HUVECs. Furthermore, C. pneumoniae infection was observed to increase the levels of total cholesterol and cholesteryl esters in LDL-treated HUVECs. In addition, C. pneumoniae was found to upregulate the expression of scavenger receptor A, cluster of differentiation 36 and acyl-coenzyme A: cholesterol acyltransferase 1 mRNA and protein. C. pneumoniae Was also observed to downregulate the mRNA and protein expression of ATP binding cassette transporter (ABC) A1 and ABCG1 in LDL-treated HUVECs. These results show that C. pneumoniae disrupts lipid metabolism in HUVECs.
引用
收藏
页码：1150 / 1156
页数：7
相关论文
共 41 条
[1]   Scavenger Receptors of Endothelial Cells Mediate the Uptake and Cellular Proatherogenic Effects of Carbamylated LDL [J].
Apostolov, Eugene O. ;
Shah, Sudhir V. ;
Ray, Debarti ;
Basnakian, Alexei G. .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2009, 29 (10) :1622-U532
[2]   Atheroprotective effects of high-density lipoproteins [J].
Assmann, G ;
Nofer, JR .
ANNUAL REVIEW OF MEDICINE, 2003, 54 :321-341
[3]   PURIFICATION AND PARTIAL CHARACTERIZATION OF THE MAJOR OUTER-MEMBRANE PROTEIN OF CHLAMYDIA-TRACHOMATIS [J].
CALDWELL, HD ;
KROMHOUT, J ;
SCHACHTER, J .
INFECTION AND IMMUNITY, 1981, 31 (03) :1161-1176
[4]   Acyl-coenzyme A: cholesterol acyltransferases [J].
Chang, Ta-Yuan ;
Li, Bo-Liang ;
Chang, Catherine C. Y. ;
Urano, Yasuomi .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2009, 297 (01) :E1-E9
[5]   Acyl-coenzyme A: Cholesterol acyltransferase [J].
Chang, TY ;
Chang, CCY ;
Cheng, D .
ANNUAL REVIEW OF BIOCHEMISTRY, 1997, 66 :613-638
[6]   Transforming growth factor-β1 modulates oxidatively modified LDL-induced expression of adhesion molecules -: Role of LOX-1 [J].
Chen, HJ ;
Li, DY ;
Saldeen, T ;
Mehta, JL .
CIRCULATION RESEARCH, 2001, 89 (12) :1155-1160
[7]   Effects of extensively oxidized low-density lipoprotein on mitochondrial function and reactive oxygen species in porcine aortic endothelial cells [J].
Chowdhury, Subir K. Roy ;
Sangle, Ganesh V. ;
Xie, Xueping ;
Stelmack, Gerald L. ;
Halayko, Andrew J. ;
Shen, Garry X. .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2010, 298 (01) :E89-E98
[8]   CD36 and macrophages in atherosclerosis [J].
Collot-Teixeira, Sophie ;
Martin, Juliette ;
McDennott-Roe, Chris ;
Poston, Robin ;
McGregor, John Louis .
CARDIOVASCULAR RESEARCH, 2007, 75 (03) :468-477
[9]   The binding of oxidized low density lipoprotein (ox-LDL) to ox-LDL receptor-1 reduces the intracellular concentration of nitric oxide in endothelial cells through an increased production of superoxide [J].
Cominacini, L ;
Rigoni, A ;
Fratta Pasini, A ;
Garbin, U ;
Davoli, A ;
Campagnola, R ;
Pastorino, AM ;
Lo Cascio, V ;
Sawamura, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (17) :13750-13755
[10]  
de Boer OJ, 2000, J PATHOL, V190, P237, DOI 10.1002/(SICI)1096-9896(200002)190:3<237::AID-PATH541>3.0.CO
12345