Antiparasitic and immunomodulatory potential of oral nanocapsules encapsulated lactoferrin protein against Plasmodium berghei

被引：15

作者：

Anand, Namrata ^{[1
]}

Kanwar, Rupinder K. ^{[2
]}

Sehgal, Rakesh ^{[1
]}

Kanwar, Jagat R. ^{[2
]}

机构：

[1] Postgrad Inst Med Educ & Res, Dept Med Parasitol, Chandigarh 160014, India

[2] Deakin Univ, Fac Hlth, C MMR, SoM,NLIMBR, Geelong, Vic 3217, Australia

来源：

NANOMEDICINE | 2016年 / 11卷 / 01期

关键词：

encapsulation; lactoferrin; miRNA; nanocapsules; Plasmodium berghei; BOVINE LACTOFERRIN; FALCIPARUM; MALARIA; OXIDE; MILK; NANOCARRIERS; TRANSFERRIN; CHLOROQUINE; INHIBITION; EXPRESSION;

D O I：

10.2217/nnm.15.181

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: To analyze the effect of native buffalo lactoferrin (buLf) protein along with its nanoformulation using alginate-enclosed, chitosan-conjugated, calcium phosphate buffalo Lf nanocapsules (AEC-CCo-CP-buLf NCs) against rodent parasite Plasmodium berghei. Materials & methods: BALB/c mice were infected with malaria parasite and efficacy of the proteins (buLf and NCs) was evaluated by measuring parasitemia, initialization, role of miRNA in absorption of NCs, parasite load by histopathology and quantitative determination, cytokine levels, bioavailability and immunohistochemistry to localize Lf protein. Results: NCs significantly reduced the parasite load in mice compared with buLf and untreated group. NCs were found to be modulating the disease profile of mice as shown by immunohistochemistry, free radical ion production and higher survival tendency. Conclusion: Our study confirms that NCs internalized and changed the expression of miRNAs that further enhanced their uptake in various organs leading to inhibitory effect against the parasite as well as maintenance of the Fe metabolism.

引用

页码：47 / 62

页数：16

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