A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus

被引：13

作者：

Wang, Weihong ^{[1
,2
]}

Park, Kyu-Hyung ^{[1
]}

Lee, Jusung ^{[1
]}

Oh, Eunseok ^{[1
]}

Park, Chanyoon ^{[3
]}

Kang, Eunmo ^{[1
]}

Lee, Juni ^{[1
]}

Kang, Heonjoong ^{[1
,2
,3
]}

机构：

[1] Seoul Natl Univ, Sch Earth & Environm Sci, Lab Marine Drugs, NS 80, Seoul 08826, South Korea

[2] Seoul Natl Univ, Res Inst Oceanog, NS 80, Seoul 08826, South Korea

[3] Seoul Natl Univ, Interdisciplinary Grad Program Genet Engn, NS 80, Seoul 08826, South Korea

来源：

MOLECULES | 2020年 / 25卷 / 19期

基金：

新加坡国家研究基金会;

关键词：

Bacillus stratosphericus; thiopeptide; antibiotic; antibacterial activity; PEPTIDE;

D O I：

10.3390/molecules25194383

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new thiopeptide (micrococcin P3, 1) and a known one (micrococcin P1, 2) were isolated from the culture broth of a marine-derived strain of Bacillus stratosphericus. The structures of both compounds were elucidated using spectroscopic methods, including extensive 1D and 2D NMR analysis, high resolution mass spectrometry (HRMS), and tandem mass spectrometry. Both compounds exhibited potent antibacterial activities against Gram-positive strains with minimum inhibitory concentration (MIC) values of 0.05-0.8 mu g/mL and did not show cytotoxicity in the MTT assay up to a concentration of 10 mu M. This study adds a new promising member, micrococcin P3, to the family of thiopeptide antibiotics, which shows potential for the development of new antibiotics targeting Gram-positive bacteria.

引用

页数：9

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