Loss of LDOC1 Expression by Promoter Methylation in Cervical Cancer Cells

被引：21

作者：

Buchholtz, Marie-Luise ^{[1
]}

Jueckstock, Julia ^{[1
]}

Weber, Elena ^{[1
]}

Mylonas, Ioannis ^{[1
]}

Dian, Darius ^{[1
]}

Bruening, Ansgar ^{[1
]}

机构：

[1] Univ Munich, Dept Obstet & Gynecol, Munich, Germany

来源：

CANCER INVESTIGATION | 2013年 / 31卷 / 09期

关键词：

LDOC1; Cervical cancer; Promoter methylation; Epigenetics; Tumor suppressor; GENE-EXPRESSION; IDENTIFICATION; PROTEIN;

D O I：

10.3109/07357907.2013.845671

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cervical cancer lacks reliable prognostic factors for both progression and chemotherapeutic responsiveness. The expression of the LDOC1 tumor suppressor candidate was therefore investigated. In four of six cervical cancer cell lines tested, expression of LDOC1 was silenced. Downregulation of LDOC1 could also be shown in biopsies of cervical cancer specimens. PCR-based promotermethylation analysis revealed a significant association between promoter methylation and the loss of LDOC1 expression, which could be reverted by DNA methyltransferase inhibitors. This indicates that silencing of LDOC1 is a frequent event in cervical cancer and may be of interest as amolecular marker in cervical cancer.

引用

页码：571 / 577

页数：7

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