Hsa-Let-7g miRNA Targets Caspase-3 and Inhibits the Apoptosis Induced by ox-LDL in Endothelial Cells

被引：49

作者：

Zhang, Yefei ^{[1
]}

Chen, Naiyun ^{[1
]}

Zhang, Jihao ^{[1
]}

Tong, Yaling ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Emergency Dept, Hangzhou 310003, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2013年 / 14卷 / 11期

关键词：

hsa-let-7g; caspase-3; apoptosis; ox-LDL; endothelial cells; SMOOTH-MUSCLE-CELLS; NF-KAPPA-B; OXIDIZED LDL; UP-REGULATION; MICRORNA EXPRESSION; LOX-1; ANGIOGENESIS; ACTIVATION; RECEPTOR-1; DEATH;

D O I：

10.3390/ijms141122708

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been well confirmed ox-LDL plays key roles in the development of atherosclerosis via binding to LOX-1 and inducing apoptosis in vascular endothelial cells. Recent studies have shown ox-LDL can suppress microRNA has-let-7g, which in turn inhibits the ox-LDL induced apoptosis. However, details need to be uncovered. To determine the anti-atherosclerosis effect of microRNA has-let-7g, and to evaluate the possibility of CASP3 as an anti-atherosclerotic drug target by has-let-7g, the present study determined the role of hsa-let-7g miRNA in ox-LDL induced apoptosis in the vascular endothelial cells. We found that miRNA has-let-7g was suppressed during the ox-LDL-induced apoptosis in EAhy926 endothelial cells. In addition, overexpression of has-let-7g negatively regulated apoptosis in the endothelial cells by targeting caspase-3 expression. Therefore, miRNA let-7g may play important role in endothelial apoptosis and atherosclerosis.

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收藏

页码：22708 / 22720

页数：13

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