Optimized Extraction Technology for Chlorogenic Acid from Sweet Potato Leaf by CCD/RSM

The aim of this study was to optimized extraction chlorogenic acid from sweet potato leave by central composite design/response surface method (CCD/RSM). The effects of ethanol concentration, ratio of solid to liquid, and extraction time on the yield of chlorogenic acid were evaluated, and the yield of chlorogenic acid under analysis optimum condition was also forecasted. The optimized extraction conditions were as follows: extraction with a 20 fold volume of 70% aqueous ethanol solution for 2h. Under these conditions the theoretical yield of chlorogenic acid was 15.95 mg/g, and the measured value was 15.89 mg/g. This method is scientific, reasonable and feasible. It also laid a foundation for research and application of chlorogenic acid in sweet potato leaves.