Characteristics and frequency of DEL phenotype detected by indirect antiglobulin test in Dalmatia county of Croatia

被引:8
|
作者
Dajak, Slavica [1 ]
Krstic, Jelena Lukacevic [1 ]
Koermoeczi, Guenther [2 ]
Dogic, Vesna [3 ]
Burilovic, Vedrana [1 ]
机构
[1] Split Univ, Ctr Hosp, Dept Transfus Med, Split 21000, Croatia
[2] Med Univ Vienna, Dept Blood Grp Serol & Transfus Med, Vienna, Austria
[3] Croatian Inst Transfus Med, Zagreb, Croatia
关键词
Weak D variants; Indirect antiglobulin test; Genotyping RHD; ANTI-D IMMUNIZATION; D-BLOOD-DONORS; RHD GENE; D-EL; INDIVIDUALS; ALLELES;
D O I
10.1016/j.transci.2014.01.019
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
D variants, collectively called DEL, express trace amounts of D antigen which is considered to be serologically detectable only by adsorption-elution techniques. We detected six cases of DEL phenotype by indirect antiglobulin test, in Dalmatia County of Croatia by routine serological testing of D antigen of new blood donors. RH genotyping found that all six donors carry allele RHD(M295I), RH genotype CcDdee. D antigen densities of D variants were very low, between 26 and 44 D antigens per red blood cell. The frequency of D variants detected by IAT allele RHD(M295I) was 1:272 in D negative donors. Obviously, DEL phenotype is more common in some parts of European population than initially thought. In conclusion, our routine serological testing of D antigen can detect extremely weak D antigen, even RBCs with DEL phenotype and antigen density as low as 26 D antigens per RBC. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:210 / 213
页数:4
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