Characteristics and frequency of DEL phenotype detected by indirect antiglobulin test in Dalmatia county of Croatia

被引：10

作者：

Dajak, Slavica ^{[1
]}

Krstic, Jelena Lukacevic ^{[1
]}

Koermoeczi, Guenther ^{[2
]}

Dogic, Vesna ^{[3
]}

Burilovic, Vedrana ^{[1
]}

机构：

[1] Split Univ, Ctr Hosp, Dept Transfus Med, Split 21000, Croatia

[2] Med Univ Vienna, Dept Blood Grp Serol & Transfus Med, Vienna, Austria

[3] Croatian Inst Transfus Med, Zagreb, Croatia

来源：

TRANSFUSION AND APHERESIS SCIENCE | 2014年 / 50卷 / 02期

关键词：

Weak D variants; Indirect antiglobulin test; Genotyping RHD; ANTI-D IMMUNIZATION; D-BLOOD-DONORS; RHD GENE; D-EL; INDIVIDUALS; ALLELES;

D O I：

10.1016/j.transci.2014.01.019

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

D variants, collectively called DEL, express trace amounts of D antigen which is considered to be serologically detectable only by adsorption-elution techniques. We detected six cases of DEL phenotype by indirect antiglobulin test, in Dalmatia County of Croatia by routine serological testing of D antigen of new blood donors. RH genotyping found that all six donors carry allele RHD(M295I), RH genotype CcDdee. D antigen densities of D variants were very low, between 26 and 44 D antigens per red blood cell. The frequency of D variants detected by IAT allele RHD(M295I) was 1:272 in D negative donors. Obviously, DEL phenotype is more common in some parts of European population than initially thought. In conclusion, our routine serological testing of D antigen can detect extremely weak D antigen, even RBCs with DEL phenotype and antigen density as low as 26 D antigens per RBC. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：210 / 213

页数：4

共 24 条

[1] RHD positive among C/E plus and D- blood donors in Denmark [J].

Christiansen, Mette ;

Sorensen, Betina S. ;

Grunnet, Niels .

TRANSFUSION, 2010, 50 (07) :1460-1464

[2]

Daniels G.L., 2002, HUMAN BLOOD GROUPS

[3] Distribution of weak D types in the Croatian population [J].

Dogic, V. ;

Bingulac-Popovic, J. ;

Babic, I. ;

Hundric-Haspl, Z. ;

Jurakovic-Loncar, N. ;

Mratinovic-Mikulandra, J. ;

Vuk, T. ;

Balija, M. ;

Jukic, I. .

TRANSFUSION MEDICINE, 2011, 21 (04) :278-279

[4]

Engelfriet CP, 2006, VOX SANG, V90, P140

[5] Homing in on D antigen immunogenicity [J].

Flegel, WA .

TRANSFUSION, 2005, 45 (04) :466-468

[6] Section 1B:: Rh flow cytometry -: Coordinator's report.: Rhesus index and antigen density:: an analysis of the reproducibility of flow cytometric determination [J].

Flegel, WA ;

Curin-Serbec, V ;

Delamaire, M ;

Donvito, B ;

Ikeda, H ;

Jorgensen, J ;

Kumpel, B ;

Le Pennec, PY ;

Písacka, M ;

Tani, Y ;

Uchikawa, M ;

Wendel, S ;

Wagner, FF .

TRANSFUSION CLINIQUE ET BIOLOGIQUE, 2002, 9 (01) :33-42

[7] Six years' experience performing RHD genotyping to confirm D- red blood cell units in Germany for preventing anti-D immunizations [J].

Flegel, Willy A. ;

von Zabern, Inge ;

Wagner, Franz F. .

TRANSFUSION, 2009, 49 (03) :465-471

[8] Presence of RHD in serologically D-, C/E+ individuals:: a European multicenter study [J].

Gassner, C ;

Doescher, A ;

Drnovsek, TD ;

Rozman, P ;

Eicher, NI ;

Legler, TJ ;

Lukin, S ;

Garritsen, H ;

Kleinrath, T ;

Egger, B ;

Ehling, R ;

Körmöczi, GF ;

Kilga-Nogler, S ;

Schoenitzer, D ;

Petershofen, EK .

TRANSFUSION, 2005, 45 (04) :527-538

[9]

Issit P.D., 1998, Applied Blood Group Serology, V4th

[10] Primary anti-D Immunization by DEL Red Blood Cells [J].

Kim, Kyeong-Hee ;

Kim, Kyung-Eun ;

Woo, Kwang-Sook ;

Han, Jin-Yeong ;

Kim, Jeong-Man ;

Park, Kyoung Un .

KOREAN JOURNAL OF LABORATORY MEDICINE, 2009, 29 (04) :361-365

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