DNA-based analysis of planktonic methanotrophs in a stratified lake

被引：27

作者：

Kojima, Hisaya ^{[1
]}

Iwata, Tomoya ^{[2
]}

Fukui, Manabu

机构：

[1] Hokkaido Univ, Inst Low Temp Sci, Kita Ku, Sapporo, Hokkaido 0600819, Japan

[2] Univ Yamanashi, Dept Ecosocial Syst Engn, Kofu, Yamanashi, Japan

来源：

FRESHWATER BIOLOGY | 2009年 / 54卷 / 07期

关键词：

community structure; freshwater lake; planktonic methanotrophs; METHANE-OXIDIZING BACTERIA; PELAGIC FOOD WEBS; MOLECULAR CHARACTERIZATION; ATMOSPHERIC METHANE; COMMUNITY STRUCTURE; SEQUENCE ALIGNMENT; CHIRONOMID LARVAE; FRENCH-GUIANA; FOREST SOILS; PETIT SAUT;

D O I：

10.1111/j.1365-2427.2009.02199.x

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

1. The assemblage of planktonic methanotrophs in a stratified freshwater lake was investigated. Vertical patterns were analysed by denaturing gradient gel electrophoresis, using the primer pair specific for 16S rRNA genes of type I methanotrophs. 2. The resulting banding patterns could be divided into three distinct groups, and sequenced bands were all related to the Methylobacter species. No amplicon was obtained with the primer pair specific for type II methanotrophs. 3. Cloning analysis of the pmoA gene was performed using samples from three water depths (epilimnion, metalimnion and hypolimnion). The compositions of the clone libraries from the three depths were distinct from each other but all three libraries were dominated by clones related to Methylobacter species.

引用

页码：1501 / 1509

页数：9

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