Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

被引：12

作者：

Lee, Jinhee ^{[1
]}

Tatsumi, Atsuro ^{[1
]}

Abe, Koichi ^{[1
]}

Yoshida, Wataru ^{[2
]}

Sode, Koji ^{[1
]}

Ikebukuro, Kazunori ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

[2] Tokyo Univ Technol, Sch Biotechnol & Biosci, Hachioji, Tokyo 1920982, Japan

来源：

ANALYTICAL METHODS | 2014年 / 6卷 / 14期

关键词：

DETECTION SYSTEM; APTAMER SANDWICH; PCR PRODUCTS; DNA; LUCIFERASE;

D O I：

10.1039/c4ay00977k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We developed an electrochemical detection system for pathogenic bacteria by utilizing a glucose dehydrogenase-fused zinc finger protein (ZF-GDH), which could detect PCR products electrochemicallywithout the need for DNA probe hybridization. Using ZF-GDH, we could specifically detect 10 copies of genomic DNA derived from Escherichia coli O157.

引用

页码：4991 / 4994

页数：4

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