Mouse Dipeptidyl Peptidase 4 Is Not a Functional Receptor for Middle East Respiratory Syndrome Coronavirus Infection

被引：75
作者：
Cockrell, Adam S. [1 ]
Peck, Kayla M. [4 ]
Yount, Boyd L. [2 ]
Agnihothram, Sudhakar S. [2 ]
Scobey, Trevor [2 ]
Curnes, Nicole R. [1 ]
Baric, Ralph S. [2 ,3 ]
Heise, Mark T. [1 ,3 ]
机构：
[1] Univ N Carolina, Dept Genet, Chapel Hill, NC 27523 USA
[2] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA
[3] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC USA
[4] Univ N Carolina, Dept Biol, Chapel Hill, NC USA
来源：
JOURNAL OF VIROLOGY | 2014年 / 88卷 / 09期
关键词：
MERS-COV; BINDING;
D O I：
10.1128/JVI.03764-13
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold, we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse-adapted MERS-CoV for rapid assessment of therapeutics.
引用
收藏
页码：5195 / 5199
页数：5
相关论文
共 11 条
  • [1] [Anonymous], 2013, PLOS CURRENTS OUTBRE
  • [2] [Anonymous], 2013, J INFECT DIS
  • [3] Wild-type and innate immune-deficient mice are not susceptible to the Middle East respiratory syndrome coronavirus
    Coleman, Christopher M.
    Matthews, Krystal L.
    Goicochea, Lindsay
    Frieman, Matthew B.
    [J]. JOURNAL OF GENERAL VIROLOGY, 2014, 95 : 408 - 412
  • [4] The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Does Not Replicate in Syrian Hamsters
    de Wit, Emmie
    Prescott, Joseph
    Baseler, Laura
    Bushmaker, Trenton
    Thomas, Tina
    Lackemeyer, Matthew G.
    Martellaro, Cynthia
    Milne-Price, Shauna
    Haddock, Elaine
    Haagmans, Bart L.
    Feldmann, Heinz
    Munster, Vincent J.
    [J]. PLOS ONE, 2013, 8 (07):
  • [5] A decade after SARS: strategies for controlling emerging coronaviruses
    Graham, Rachel L.
    Donaldson, Eric F.
    Baric, Ralph S.
    [J]. NATURE REVIEWS MICROBIOLOGY, 2013, 11 (12) : 836 - 848
  • [6] Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26
    Lu, Guangwen
    Hu, Yawei
    Wang, Qihui
    Qi, Jianxun
    Gao, Feng
    Li, Yan
    Zhang, Yanfang
    Zhang, Wei
    Yuan, Yuan
    Bao, Jinku
    Zhang, Buchang
    Shi, Yi
    Yan, Jinghua
    Gao, George F.
    [J]. NATURE, 2013, 500 (7461) : 227 - +
  • [7] Adenosine Deaminase Acts as a Natural Antagonist for Dipeptidyl Peptidase 4-Mediated Entry of the Middle East Respiratory Syndrome Coronavirus
    Raj, V. Stalin
    Smits, Saskia L.
    Provacia, Lisette B.
    van den Brand, Judith M. A.
    Wiersma, Lidewij
    Ouwendijk, Werner J. D.
    Bestebroer, Theo M.
    Spronken, Monique I.
    van Amerongen, Geert
    Rottier, Peter J. M.
    Fouchier, Ron A. M.
    Bosch, Berend Jan
    Osterhaus, Albert D. M. E.
    Haagmans, Bart L.
    [J]. JOURNAL OF VIROLOGY, 2014, 88 (03) : 1834 - 1838
  • [8] Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC
    Raj, V. Stalin
    Mou, Huihui
    Smits, Saskia L.
    Dekkers, Dick H. W.
    Mueller, Marcel A.
    Dijkman, Ronald
    Muth, Doreen
    Demmers, Jeroen A. A.
    Zaki, Ali
    Fouchier, Ron A. M.
    Thiel, Volker
    Drosten, Christian
    Rottier, Peter J. M.
    Osterhaus, Albert D. M. E.
    Bosch, Berend Jan
    Haagmans, Bart L.
    [J]. NATURE, 2013, 495 (7440) : 251 - 254
  • [9] Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus
    Scobey, Trevor
    Yount, Boyd L.
    Sims, Amy C.
    Donaldson, Eric F.
    Agnihothram, Sudhakar S.
    Menachery, Vineet D.
    Graham, Rachel L.
    Swanstrom, Jesica
    Bove, Peter F.
    Kim, Jeeho D.
    Grego, Sonia
    Randell, Scott H.
    Baric, Ralph S.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (40) : 16157 - 16162
  • [10] Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4
    Wang, Nianshuang
    Shi, Xuanling
    Jiang, Liwei
    Zhang, Senyan
    Wang, Dongli
    Tong, Pei
    Guo, Dongxing
    Fu, Lili
    Cui, Ye
    Liu, Xi
    Arledge, Kelly C.
    Chen, Ying-Hua
    Zhang, Linqi
    Wang, Xinquan
    [J]. CELL RESEARCH, 2013, 23 (08) : 986 - 993
12