Structural Characterization of a Novel Glucan from Achatina fulica and Its Antioxidant Activity

被引：51
作者：
Liao, Ningbo [1 ]
Chen, Shiguo [1 ]
Ye, Xingqian [1 ]
Zhong, Jianjun [1 ]
Ye, Xuan [3 ]
Yin, Xinzi [1 ]
Tian, Jenny [4 ]
Liu, Donghong [1 ,2 ]
机构：
[1] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Fuli Inst Food Sci, Hangzhou 310058, Zhejiang, Peoples R China
[3] Univ London Imperial Coll Sci Technol & Med, Dept Mat, Royal Sch Mines, London SW7 2BP, England
[4] McGill Univ, Dept Food Sci & Agr Chem, Montreal, PQ H9X 3V9, Canada
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2014年 / 62卷 / 11期
基金：
中国国家自然科学基金;
关键词：
glucan; Achatina fulica; structure; antioxidant activity; LYCIUM-BARBARUM POLYSACCHARIDES; SNAIL;
D O I：
10.1021/jf403896c
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A novel glucan designated AFPS-IB was purified from Achatina fulica (China white jade snail) by anion-exchange and gel-permeation chromatography. Chemical composition analysis indicated AFPS-IB was composed of glucose, fucose, rhamnose, mannose, and galactose in a molar ratio of 189:2:1:1:2 and with an average molecular weight of 128 kDa. Its structural characteristics were investigated by Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), gas chromatography mass spectrometry (GC-MS), methylation analysis, nuclear magnetic resonance (NMR) spectroscopy (H-1, C-13, H-H COSY, HSQC, TOCSY, and NOESY), and atomic force microscopy (AFM). The glucan mainly consisted of a backbone of repeating (1 -> 4)-alpha-D-glucose residues with (1 -> 6)-beta-D glucosyl branches at random points on the backbone glucose. Antioxidant studies revealed AFPS-IB showed significant DPPH (2,2-diphenyl-1-picrylhydrazyl) radical, superoxide anion (O-2(-)) scavenging activities and high reduction potential. This study suggested that AFPS-IB could be a new source of dietary antioxidants.
引用
收藏
页码：2344 / 2352
页数：9
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