In Vivo Tracking of Fluorinated Polypeptide Gene Carriers by Positron Emission Tomography Imaging

被引:21
作者
Wang, Xinyu [1 ]
Rong, Guangyu [2 ]
Yan, Junjie [1 ]
Pan, Donghui [1 ]
Wang, Lizhen [1 ]
Xu, Yuping [1 ]
Yang, Min [1 ,3 ]
Cheng, Yiyun [2 ,4 ]
机构
[1] Jiangsu Inst Nucl Med, NHC Key Lab Nucl Med, Jiangsu Key Lab Mol Nucl Med, Wuxi 214063, Jiangsu, Peoples R China
[2] East China Normal Univ, Sch Life Sci, Shanghai Key Lab Regulatory Biol, Shanghai 200241, Peoples R China
[3] Nanjing Med Univ, Sch Pharm, Dept Radiopharmaceut, Nanjing 210029, Jiangsu, Peoples R China
[4] South China Univ Technol, Sch Mol Sci & Engn, South China Adv Inst Soft Matter Sci & Technol, Guangzhou 510640, Guangdong, Peoples R China
基金
国家重点研发计划;
关键词
fluorination; polypeptide; gene transfection; radiolabelling; positron emission tomography imaging; SIRNA DELIVERY; NANOPARTICLES; POLYMERS; THERAPY; FLUOROPOLYMERS; DEFLUORINATION; DENDRIMERS; PROSPECTS; PROGRESS; PEPTIDE;
D O I
10.1021/acsami.0c11967
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Fluorinated polymers have attracted increasing attention in gene delivery and cytosolic protein delivery in recent years. In vivo tracking of fluorinated polymers will be of great importance to evaluate their biodistribution, clearance, and safety. However, tracking of polymeric carriers without changing their chemical structures remains a huge challenge. Herein, we reported a series of fluorinated poly-L-(lysine) (F-PLL) with high gene transfection efficiency and excellent biodegradation. Radionuclide F-18 was radiolabeled on F-PLL by halogen replacement without chemical modification. The radiolabeling of F-PLL offers positron emission tomography (PET) imaging for in vivo tracking of the polymers. The biodistribution of F-PLL and the DNA complexes revealed by micro-PET imaging illustrated the rapid clearance of fluorinated polymers from liver and intestine after intravenous administration. The results demonstrated that the polymer F-PLL will not be accumulated in the liver and spleen when administrated as a gene carrier. This work presents a new strategy for in vivo tracking fluorinated polymers via PET imaging.
引用
收藏
页码:45763 / 45771
页数:9
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