Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

被引：16
|
作者：
Piqueur, Marian A. C. [1 ]
Verstrepen, Walter A. [1 ]
Bruynseels, Peggy [1 ]
Mertens, An H. [1 ]
机构：
[1] ZNA Hosp, Dept Microbiol, B-2020 Antwerp, Belgium
来源：
VIROLOGY JOURNAL | 2009年 / 6卷
关键词：
CEREBROSPINAL-FLUID SPECIMENS; AMPLIFICATION;
D O I：
10.1186/1743-422X-6-95
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.
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页数：3
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