共 49 条
Generating NMR chemical shift assignments of intrinsically disordered proteins using carbon-detected NMR methods
被引:41
作者:

Sahu, Debashish
论文数: 0 引用数: 0
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机构:
Penn State Univ, Dept Chem, University Pk, PA 16802 USA Penn State Univ, Dept Chem, University Pk, PA 16802 USA

Bastidas, Monique
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Penn State Univ, Dept Chem, University Pk, PA 16802 USA Penn State Univ, Dept Chem, University Pk, PA 16802 USA

Showalter, Scott A.
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h-index: 0
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Penn State Univ, Dept Chem, University Pk, PA 16802 USA Penn State Univ, Dept Chem, University Pk, PA 16802 USA
机构:
[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
基金:
美国国家卫生研究院;
美国国家科学基金会;
关键词:
Intrinsically disordered proteins;
Carbon detect NMR;
Amino acid-filtered NMR;
CAS-NMR;
Pdx1;
INSULIN;
GENE;
TRANSCRIPTION;
MUTATIONS;
PDX1;
SPECTROSCOPY;
RECOGNITION;
COMPLEXITY;
DOMAINS;
BINDING;
D O I:
10.1016/j.ab.2013.12.005
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
There is an extraordinary need to describe the structures of intrinsically disordered proteins (IDPs) due to their role in various biological processes involved in signaling and transcription. However, general study of IDPs by NMR spectroscopy is limited by the poor H-1 amide chemical shift dispersion typically observed in their spectra. Recently, C-13 direct-detected NMR spectroscopy has been recognized as enabling broad structural study of IDPs. Most notably, multidimensional experiments based on the N-15,C-13 CON spectrum make complete chemical shift assignment feasible. Here we document a collection of NMR-based tools that efficiently lead to chemical shift assignment of IDPs, motivated by a case study of the C-terminal disordered region from the human pancreatic transcription factor Pdx1. Our strategy builds on the combination of two three-dimensional (3D) experiments, (H-N-flip)N(CA)CON and 3D (H-N-flip)N(CA)NCO, that enable daisy chain connections to be built along the IDP backbone, facilitated by acquisition of amino acid-specific N-15,C-13 CON-detected experiments. Assignments are completed through carbon-detected, total correlation spectroscopy (TOCSY)-based side chain chemical shift measurement. Conducting our study required producing valuable modifications to many previously published pulse sequences, motivating us to announce the creation of a database of our pulse programs, which we make freely available through our website. (C) 2013 Elsevier Inc. All rights reserved.
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页码:17 / 25
页数:9
相关论文
共 49 条
[1]
Update on activities at the Universal Protein Resource (UniProt) in 2013
[J].
Apweiler, Rolf
;
Martin, Maria Jesus
;
O'Donovan, Claire
;
Magrane, Michele
;
Alam-Faruque, Yasmin
;
Alpi, Emanuela
;
Antunes, Ricardo
;
Arganiska, Joanna
;
Casanova, Elisabet Barrera
;
Bely, Benoit
;
Bingley, Mark
;
Bonilla, Carlos
;
Britto, Ramona
;
Bursteinas, Borisas
;
Chan, Wei Mun
;
Chavali, Gayatri
;
Cibrian-Uhalte, Elena
;
Da Silva, Alan
;
De Giorgi, Maurizio
;
Dimmer, Emily
;
Fazzini, Francesco
;
Gane, Paul
;
Fedotov, Alexander
;
Castro, Leyla Garcia
;
Garmiri, Penelope
;
Hatton-Ellis, Emma
;
Hieta, Reija
;
Huntley, Rachael
;
Jacobsen, Julius
;
Jones, Rachel
;
Legge, Duncan
;
Liu, Wudong
;
Luo, Jie
;
MacDougall, Alistair
;
Mutowo, Prudence
;
Nightingale, Andrew
;
Orchard, Sandra
;
Patient, Samuel
;
Pichler, Klemens
;
Poggioli, Diego
;
Pundir, Sangya
;
Pureza, Luis
;
Qi, Guoying
;
Rosanoff, Steven
;
Sawford, Tony
;
Sehra, Harminder
;
Turner, Edward
;
Volynkin, Vladimir
;
Wardell, Tony
;
Watkins, Xavier
.
NUCLEIC ACIDS RESEARCH,
2013, 41 (D1)
:D43-D47

论文数: 引用数:
h-index:
机构:

Martin, Maria Jesus
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

O'Donovan, Claire
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Magrane, Michele
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Alam-Faruque, Yasmin
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Alpi, Emanuela
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Antunes, Ricardo
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Arganiska, Joanna
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Casanova, Elisabet Barrera
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Bely, Benoit
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Bingley, Mark
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Bonilla, Carlos
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Britto, Ramona
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Bursteinas, Borisas
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Chan, Wei Mun
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Chavali, Gayatri
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Cibrian-Uhalte, Elena
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Da Silva, Alan
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

De Giorgi, Maurizio
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Dimmer, Emily
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Fazzini, Francesco
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Gane, Paul
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Fedotov, Alexander
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Castro, Leyla Garcia
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Garmiri, Penelope
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Hatton-Ellis, Emma
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Hieta, Reija
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Huntley, Rachael
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Jacobsen, Julius
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Jones, Rachel
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Legge, Duncan
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Liu, Wudong
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Luo, Jie
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

MacDougall, Alistair
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Mutowo, Prudence
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Nightingale, Andrew
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Orchard, Sandra
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Patient, Samuel
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Pichler, Klemens
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Poggioli, Diego
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Pundir, Sangya
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Pureza, Luis
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Qi, Guoying
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Rosanoff, Steven
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Sawford, Tony
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Sehra, Harminder
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Turner, Edward
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Volynkin, Vladimir
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Wardell, Tony
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England

Watkins, Xavier
论文数: 0 引用数: 0
h-index: 0
机构: European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England
[2]
Thermodynamic and Structural Determinants of Differential Pdx1 Binding to Elements from the Insulin and IAPP Promoters
[J].
Bastidas, Monique
;
Showalter, Scott A.
.
JOURNAL OF MOLECULAR BIOLOGY,
2013, 425 (18)
:3360-3377

Bastidas, Monique
论文数: 0 引用数: 0
h-index: 0
机构:
Penn State Univ, Dept Chem, University Pk, PA 16802 USA Penn State Univ, Dept Chem, University Pk, PA 16802 USA

Showalter, Scott A.
论文数: 0 引用数: 0
h-index: 0
机构:
Penn State Univ, Dept Chem, University Pk, PA 16802 USA Penn State Univ, Dept Chem, University Pk, PA 16802 USA
[3]
The Protein Data Bank
[J].
Berman, HM
;
Westbrook, J
;
Feng, Z
;
Gilliland, G
;
Bhat, TN
;
Weissig, H
;
Shindyalov, IN
;
Bourne, PE
.
NUCLEIC ACIDS RESEARCH,
2000, 28 (01)
:235-242

Berman, HM
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

Westbrook, J
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

Feng, Z
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

Gilliland, G
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

Bhat, TN
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

Weissig, H
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

Shindyalov, IN
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

Bourne, PE
论文数: 0 引用数: 0
h-index: 0
机构: Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA
[4]
13C-detected protonless NMR spectroscopy of proteins in solution
[J].
Bermel, W
;
Bertini, I
;
Felli, IC
;
Piccioli, M
;
Pierattelli, R
.
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY,
2006, 48 (01)
:25-45

Bermel, W
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Bertini, I
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Felli, IC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Piccioli, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Pierattelli, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy
[5]
Exclusively Heteronuclear 13C-Detected Amino-Acid-Selective NMR Experiments for the Study of Intrinsically Disordered Proteins (IDPs)
[J].
Bermel, Wolfgang
;
Bertini, Ivano
;
Chill, Jordan
;
Felli, Isabella C.
;
Haba, Noam
;
Kumar, Vasantha M. V.
;
Pierattelli, Roberta
.
CHEMBIOCHEM,
2012, 13 (16)
:2425-2432

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Bertini, Ivano
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy
Univ Florence, Dept Chem Ugo Shiff, I-50019 Florence, Italy Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

论文数: 引用数:
h-index:
机构:

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy
Univ Florence, Dept Chem Ugo Shiff, I-50019 Florence, Italy Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Haba, Noam
论文数: 0 引用数: 0
h-index: 0
机构:
Bar Ilan Univ, Dept Chem, IL-52900 Ramat Gan, Israel Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Kumar, Vasantha M. V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy
Univ Florence, Dept Chem Ugo Shiff, I-50019 Florence, Italy Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy
[6]
Speeding up sequence specific assignment of IDPs
[J].
Bermel, Wolfgang
;
Bertini, Ivano
;
Felli, Isabella C.
;
Gonnelli, Leonardo
;
Kozminski, Wiktor
;
Piai, Alessandro
;
Pierattelli, Roberta
;
Stanek, Jan
.
JOURNAL OF BIOMOLECULAR NMR,
2012, 53 (04)
:293-301

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, Silberstreifen, D-76287 Rheinstetten, Germany Univ Florence, CERM, I-50019 Florence, Italy

Bertini, Ivano
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Gonnelli, Leonardo
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Kozminski, Wiktor
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Warsaw, Fac Chem, PL-02093 Warsaw, Poland Univ Florence, CERM, I-50019 Florence, Italy

Piai, Alessandro
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

论文数: 引用数:
h-index:
机构:
[7]
Speeding Up 13C Direct Detection Biomolecular NMR Spectroscopy
[J].
Bermel, Wolfgang
;
Bertini, Ivano
;
Felli, Isabella C.
;
Pierattelli, Roberta
.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
2009, 131 (42)
:15339-15345

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany Univ Florence, CERM, I-50019 Florence, Italy

Bertini, Ivano
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy
[8]
Pancreatic transcription factors and their role in the birth, life and survival of the pancreatic β cell
[J].
Bernardo, Andreia S.
;
Hay, Colin W.
;
Docherty, Kevin
.
MOLECULAR AND CELLULAR ENDOCRINOLOGY,
2008, 294 (1-2)
:1-9

Bernardo, Andreia S.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Aberdeen, Inst Med Sci, Sch Med Sci, Aberdeen AB25 2ZD, Scotland Univ Aberdeen, Inst Med Sci, Sch Med Sci, Aberdeen AB25 2ZD, Scotland

Hay, Colin W.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Aberdeen, Inst Med Sci, Sch Med Sci, Aberdeen AB25 2ZD, Scotland Univ Aberdeen, Inst Med Sci, Sch Med Sci, Aberdeen AB25 2ZD, Scotland

Docherty, Kevin
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Aberdeen, Inst Med Sci, Sch Med Sci, Aberdeen AB25 2ZD, Scotland Univ Aberdeen, Inst Med Sci, Sch Med Sci, Aberdeen AB25 2ZD, Scotland
[9]
High-Resolution Characterization of Intrinsic Disorder in Proteins: Expanding the Suite of 13C-Detected NMR Spectroscopy Experiments to Determine Key Observables
[J].
Bertini, Ivano
;
Felli, Isabella C.
;
Gonnelli, Leonardo
;
Kumar, Vasantha M. V.
;
Pierattelli, Roberta
.
CHEMBIOCHEM,
2011, 12 (15)
:2347-2352

Bertini, Ivano
论文数: 0 引用数: 0
h-index: 0
机构:
CERM Univ Florence, I-50019 Sesto Fiorentino, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Sesto Fiorentino, Italy CERM Univ Florence, I-50019 Sesto Fiorentino, Italy

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
CERM Univ Florence, I-50019 Sesto Fiorentino, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Sesto Fiorentino, Italy CERM Univ Florence, I-50019 Sesto Fiorentino, Italy

Gonnelli, Leonardo
论文数: 0 引用数: 0
h-index: 0
机构:
CERM Univ Florence, I-50019 Sesto Fiorentino, Italy CERM Univ Florence, I-50019 Sesto Fiorentino, Italy

Kumar, Vasantha M. V.
论文数: 0 引用数: 0
h-index: 0
机构:
CERM Univ Florence, I-50019 Sesto Fiorentino, Italy CERM Univ Florence, I-50019 Sesto Fiorentino, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
CERM Univ Florence, I-50019 Sesto Fiorentino, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Sesto Fiorentino, Italy CERM Univ Florence, I-50019 Sesto Fiorentino, Italy
[10]
EXPERIMENTAL ASPECTS OF CHIRP NMR-SPECTROSCOPY
[J].
BOHLEN, JM
;
BODENHAUSEN, G
.
JOURNAL OF MAGNETIC RESONANCE SERIES A,
1993, 102 (03)
:293-301

BOHLEN, JM
论文数: 0 引用数: 0
h-index: 0
机构: Section de Chimie, Universitá de Lausanne, CH-1005 Lausanne

BODENHAUSEN, G
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机构: Section de Chimie, Universitá de Lausanne, CH-1005 Lausanne