Magnesium lithospermate B acts against dextran sodiumsulfate-induced ulcerative colitis by inhibiting activation of the NRLP3/ASC/Caspase-1 pathway

被引:20
作者
Jiang, Xiaoyun [1 ]
Zhong, Liang [1 ]
Sun, Dayu [1 ]
Rong, Lan [1 ]
机构
[1] Fudan Univ, Huashan Hosp, Dept Gastroenterol, Shanghai 200433, Peoples R China
关键词
Magnesium lithospermate B; Ulcerative colitis; Tumor necrosis factor-alpha; NOD-like receptor protein; Apoptosis-associated speck-like protein containing; Caspase-1; INFLAMMATORY-BOWEL-DISEASE; SULFATE SODIUM DSS; NOD-LIKE RECEPTORS; INTESTINAL INFLAMMATION; NLRP3; INFLAMMASOME; CROHNS-DISEASE; MICE; RATS; PATHOGENESIS; COAGULATION;
D O I
10.1016/j.etap.2015.10.009
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
This study aimed to observe the therapeutic effects of magnesium lithospermate B on acute and chronic colitis induced by dextran sodiumsulfate (DSS) and the role of inflammasome complex (NOD-like receptor protein, NLRP; apoptosis-associated speck-like protein containing, ASC; caspase-1). Establishment of acute and chronic colitis models were by using 5% DSS oral administration in BALB/C male mice. Magnesium lithospermate B (240 mg/kg body weight) was given by subcutaneous injection. Samples were collected for biomarker assay, histological examination, immunohistochemical evaluation and western blot. There was obvious increase in TNF-alpha level and NLPR3, ASC, and caspase-1 expressions in acute and chronic colitis groups compared with the normal control. Significant decrease of the tumor necrosis factor-alpha level and the expressions of NLPR3, ASC, and caspase-1 were observed after treatment with magnesium lithospermate B. This study showed that magnesium lithospermate B could be used to treat acute and chronic colitis by inhibiting the activation of the NLRP3/ASC/Caspase-1 pathway. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:72 / 77
页数:6
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