The incorporation of nucleoside analogs by human immunodeficiency virus type 1 reverse transcriptase decreases in the presence of polyamines

被引：2

作者：

Bakhanashvili, Mary ^{[1
]}

Rahav, Galia

机构：

[1] Chaim Sheba Med Ctr, Infect Dis Unit, IL-52621 Tel Hashomer, Ramat Gan, Israel

[2] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, Ramat Gan, Israel

来源：

FEBS LETTERS | 2006年 / 580卷 / 22期

关键词：

HIV-1; reverse transcriptase; nucleoside analogs; DNA synthesis; polyamines;

D O I：

10.1016/j.febslet.2006.08.074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleoside analogs (NAs) are an important class of anti-retroviral compounds used against human immunodeficiency virus (HIV). We have analyzed the potential effect of polyamines on the incorporation of NAs during DNA synthesis by HIV type-1 (HIV-1) reverse transcriptase (RT). The polyamines exert the ability to decrease the incorporation of various dideoxynucleoside triphosphates (ddATP, ddTTP or ddCTP) with both RNA/DNA and DNA/DNA substrates in the following order: spermine > spermidine > putrescine. The reduction is a sequence-independent effect, taking place at different sequence context. The results suggest that polyamines might affect the inhibition of reverse transcription by nucleoside analogs HIV-1 RT directed. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：5363 / 5370

页数：8

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