Occurrence and characterisation of the hydrogen-evolving enzyme in Frankia sp.

被引：1
|
作者：
Mohapatra, A.
Leul, M.
Sandström, G.
Sellstedt, A. [1 ]
机构：
[1] Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden
[2] Karelinska Univ Hosp, Div Clin Bacteriol, Dept Lab Med, Karolinska Inst, S-14186 Stockholm, Sweden
来源：
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2006年 / 31卷 / 11期
关键词：
Frankia; hydrogen; hydrogen-evolving hydrogenase; nickel;
D O I：
10.1016/j.ijhydene.2006.06.009
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
An increase in hydrogen evolution from the hydrogen-evolving enzyme in the actinomycete Frankia was recorded in the presence of nickel. Immunogold localisation analysis of the intracellular distribution of hydrogenase proteins indicated that they were evenly distributed in the membranes and cytosol of both hyphae and vesicles. In addition, molecular characterisation of the hydrogen-evolving enzyme at the proteomic level, using two-dimensional gel electrophoresis combined with mass spectrometry, confirmed that the Frankia hydrogen-evolving enzyme is similar to the cyanobacterial bidirectional hydrogenase of Anabena siamensis. (c) 2006 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：1445 / 1451
页数：7
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