Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis mosquito-larvicidal Cry4Aa mutant toxin

被引：9
|
作者：
Boonserm, P
Angsuthanasombat, C
Lescar, J
机构：
[1] Mahidol Univ, Inst Mol Biol & Genet, Nakhon Pathom 73170, Thailand
[2] Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷
关键词：
D O I：
10.1107/S0907444904011205
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The 65 kDa functional form of the mosquito-larvicidal Cry4Aa-R235Q mutant toxin has been crystallized. The crystals belong to space group C222(1), with unit-cell parameters a = 91.2, b = 202.1, c = 98.7 Angstrom, and contain one molecule per asymmetric unit. The crystals diffract to similar to2.9 Angstrom using synchrotron radiation and a complete native data set has been collected. The structure has been solved using a molecular-replacement method with the Cry4Ba toxin protein as a search model.
引用
收藏
页码：1315 / 1318
页数：4
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