Bacteria transfer by deformation through microfiltration membrane

被引：60

作者：

Gaveau, Arthur ^{[1
]}

Coetsier, Clemence ^{[1
]}

Roques, Christine ^{[1
]}

Bacchin, Patrice ^{[1
]}

Dague, Etienne ^{[2
]}

Causserand, Christel ^{[1
]}

机构：

[1] Univ Toulouse, Lab Genie Chim, CNRS, INPT,UPS, Toulouse, France

[2] CNRS, LAAS, Toulouse, France

来源：

JOURNAL OF MEMBRANE SCIENCE | 2017年 / 523卷

关键词：

Microfiltration; Bacteria; Transfer; Deformable particles; Membrane selectivity; ATOMIC-FORCE MICROSCOPY; ESCHERICHIA-COLI; PSEUDOMONAS-AERUGINOSA; CYLINDRICAL PORES; CELL-DEFORMATION; SIZE; ANTIBIOTICS; RETENTION; FILTERS; MODEL;

D O I：

10.1016/j.memsci.2016.10.023

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Living particles such as bacteria are able to transfer through membrane pores that are smaller than cell size due to the specific stiffness of this type of microorganism. This phenomenon can lead to a significant loss of selectivity in the filtration process, which is a major cause of concern in the sterilizing filtration step. This study investigates the retention of three bacteria strains: Escherichia coli CIP 54124, Pseudomonas aeruginosa CIP 103467 and Staphylococcus aureus CIP 53154 by model porous membranes for various operating conditions (transmembrane pressure, feed concentration and the physicochemical composition of filtered media with antibacterial agent added at sublethal concentration). The first part of this study is dedicated to defining the size and the nanomechanical properties of the envelope of the studied bacteria by microscopic techniques (Transmission electron microscopy & Atomic-force microscopy), in order to then explore the role of these quantifiable characteristics on the cell transfer through the pores by deformation mechanisms. Our results lead to the development of a numerical model to connect the observed retention efficiency of the filtration experiment and the microscopic information about individual particles.

引用

页码：446 / 455

页数：10

共 38 条

[1] Determination of the red blood cell ability to traverse cylindrical pores [J].

Abatti, PJ .

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 1997, 44 (03) :209-212

[2]

[Anonymous], 2013, F83805 ASTM

[3] CHANGES IN BUOYANT DENSITY AND CELL-SIZE OF ESCHERICHIA-COLI IN RESPONSE TO OSMOTIC SHOCKS [J].

BALDWIN, WW ;

SHEU, MJT ;

BANKSTON, PW ;

WOLDRINGH, CL .

JOURNAL OF BACTERIOLOGY, 1988, 170 (01) :452-455

[4] Bacterial cell curvature through mechanical control of cell growth [J].

Cabeen, Matthew T. ;

Charbon, Godefroid ;

Vollmer, Waldemar ;

Born, Petra ;

Ausmees, Nora ;

Weibel, Douglas B. ;

Jacobs-Wagner, Christine .

EMBO JOURNAL, 2009, 28 (09) :1208-1219

[5] Cell Size Control in Bacteria [J].

Chien, An-Chun ;

Hill, Norbert S. ;

Levin, Petra Anne .

CURRENT BIOLOGY, 2012, 22 (09) :R340-R349

[6]

Cronholm Karen, 2009, PDA J Pharm Sci Technol, V63, P438

[7] Assembly of live micro-organisms on microstructured PDMS stamps by convective/capillary deposition for AFM bio-experiments [J].

Dague, E. ;

Jauvert, E. ;

Laplatine, L. ;

Viallet, B. ;

Thibault, C. ;

Ressier, L. .

NANOTECHNOLOGY, 2011, 22 (39)

[8] Microbial nanoscopy: a closer look at microbial cell surfaces [J].

Dupres, Vincent ;

Alsteens, David ;

Andre, Guillaume ;

Dufrene, Yves F. .

TRENDS IN MICROBIOLOGY, 2010, 18 (09) :397-405

[9] Nutritional effects of culture media on mycoplasma cell size and removal by filtration [J].

Folmsbee, Martha ;

Howard, Glenn ;

McAlister, Morven .

BIOLOGICALS, 2010, 38 (02) :214-217

[10] Nanoscale analysis of the effects of antibiotics and CX1 on a Pseudomonas aeruginosa multidrug- resistant strain [J].

Formosa, C. ;

Grare, M. ;

Jauvert, E. ;

Coutable, A. ;

Regnouf-de-Vains, J. B. ;

Mourer, M. ;

Duval, R. E. ;

Dague, E. .

SCIENTIFIC REPORTS, 2012, 2

← 1 2 3 4 →