Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system

被引：42

作者：

Park, Kyungtae ^{[1
]}

Jeong, Hyejoong ^{[1
]}

Tanum, Junjira ^{[1
]}

Yoo, Jae-chan ^{[2
,3
]}

Hong, Jinkee ^{[1
]}

机构：

[1] Yonsei Univ, Sch Chem & Biomol Engn, 50 Yonsei Ro, Seoul 03722, South Korea

[2] Yonsei Univ, Biotechnol Res Ctr, JCBIO Co LTD, Seoul 03722, South Korea

[3] Yonsei Univ, Avison Biomed Res Ctr ABMRC, Seoul 03722, South Korea

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

基金：

新加坡国家研究基金会;

关键词：

HYDROPHOBIC INTERACTION; CELL-PROLIFERATION; RELEASE; ANGIOGENESIS; POLYPEPTIDES; FABRICATION; PHYSIOLOGY; CAPSULES; MEDIATOR; GROWTH;

D O I：

10.1038/s41598-019-44678-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To utilize potentials of nitric oxide (NO) gas in anti-bacterial, anticancer, wound healing applications, numerous studies have been conducted to develop a NO delivery system in the past few decades. Even though a coating method and film types are essential to apply in biomedical device coating from previous NO delivery systems, release control from the coating system is still challenging. In this study, we introduced a multilayered polymeric coating system to overcome the uncontrollable NO release kinetics of film systems. We used biocompatible gelatin and tannic acid to construct a rough, porous structured film based on the layer-by-layer self-assembly method. The multilayered polymeric structure facilitated the controlled amount of NO release from (Gel/TA)(n) film and showed burst release in early period owing to their large surface area from the rough, porous structure. We synthesized the proton-responsive NO donor, N-diazeniumdiolate (NONOates), into the (Gel/TA)(n) film through a chemical reaction under high pressure NO gas. NO release profile was analyzed by a real-time NO analysis machine (NOA 280i). Then, the NO-releasing (Gel/TA)(n) film was tested its toxicity against human dermal fibroblast cells and bactericidal effects against Staphylococcus aureus.

引用

页数：8