Rab35 regulates neurite outgrowth and cell shape

被引：77

作者：

Chevallier, Julien ^{[1
]}

Koop, Charles ^{[1
]}

Srivastava, Archana ^{[1
]}

Petrie, Ryan J. ^{[1
]}

Lamarche-Vane, Nathalie ^{[1
]}

Presley, John F. ^{[1
]}

机构：

[1] McGill Univ, Dept Anat & Cell Biol, Montreal, PQ H3A 2B2, Canada

来源：

FEBS LETTERS | 2009年 / 583卷 / 07期

基金：

瑞士国家科学基金会;

关键词：

Rab35; Neurite growth; Cell shape; Actin; Small GTPase; Rac1; RhoA; Cdc42; RHO-GTPASES; IN-VIVO; RECEPTOR; CYTOKINESIS; TRAFFICKING;

D O I：

10.1016/j.febslet.2009.03.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies have identified Rab35 in the endocytic pathway and as a regulator of cytokinesis; however its molecular mechanisms are currently unknown. Here, we find that Rab35 colocalizes with actin. laments and with Cdc42, Rac1 and RhoA, and that Rab35 can activate Cdc42 both in vivo and in vitro. We find activated Rab35 stimulates neurite outgrowth in PC12 and N1E-115 cells via a Cdc42-dependent pathway and that siRNA knockdown of Rab35 activity abolishes neurite outgrowth in these cell lines. We conclude that one function of Rab35 is to regulate Rho-family GTPases and that this role has consequences for neurite outgrowth.

引用

页码：1096 / 1101

页数：6

共 19 条

[11] Rac1 and Cdc42 but not RhoA or Rho kinase activities are required for neurite outgrowth induced by the netrin-1 receptor DCC (Deleted in Colorectal Cancer) in N1E-115 neuroblastoma cells
Li, XD
Saint-Cyr-Proulx, E
Aktories, K
Lamarche-Vane, N
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (17) : 15207 - 15214
[12] Export from pericentriolar endocytic recycling compartment to cell surface depends on stable, detyrosinated (Glu) microtubules and kinesin
Lin, SX
Gundersen, GG
Maxfield, FR
[J]. MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (01) : 96 - 109
[13] The role of Rho GTPases and associated kinases in regulating neurite outgrowth
Nikolic, M
[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2002, 34 (07) : 731 - 745
[14] Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunological synapse formation
Patino-Lopez, Genaro
Dong, Xiaoyun
Ben-Aissa, Khadija
Bernot, Kelsie M.
Itoh, Takashi
Fukuda, Mitsunori
Kruhlak, Michael J.
Samelson, Lawrence E.
Shaw, Stephen
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (26) : 18323 - 18330
[15] Re-evaluation of primary structure, topology, and localization of Scamper, a putative intracellular Ca2+ channel activated by sphingosylphosphocholine
Schnurbus, R
Tonelli, DD
Grohovaz, F
Zacchetti, D
[J]. BIOCHEMICAL JOURNAL, 2002, 362 (01) : 183 - 189
[16] Rab27a is an essential component of melanosome receptor for myosin Va
Wu, XF
Wang, F
Rao, K
Sellers, JR
Hammer, JA
[J]. MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (05) : 1735 - 1749
[17] YANG CZ, 1994, J BIOL CHEM, V269, P31891
[18] NGF-induced Tyro3 and Axl function as survival factors for differentiating PC12 cells
Zheng, Yan
Zhang, Lijuan
Lu, Qingjun
Wang, Xuan
Yu, Fen
Wang, Xiaomin
Lu, Qingxian
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 378 (03) : 371 - 375
[19] CB1 cannabinoid receptor-mediated neurite remodeling in mouse neuroblastoma N1E-115 cells
Zhou, D
Song, ZH
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2001, 65 (04) : 346 - 353

← 1 2 →