Rab35 regulates neurite outgrowth and cell shape

被引：77

作者：

Chevallier, Julien ^{[1
]}

Koop, Charles ^{[1
]}

Srivastava, Archana ^{[1
]}

Petrie, Ryan J. ^{[1
]}

Lamarche-Vane, Nathalie ^{[1
]}

Presley, John F. ^{[1
]}

机构：

[1] McGill Univ, Dept Anat & Cell Biol, Montreal, PQ H3A 2B2, Canada

来源：

FEBS LETTERS | 2009年 / 583卷 / 07期

基金：

瑞士国家科学基金会;

关键词：

Rab35; Neurite growth; Cell shape; Actin; Small GTPase; Rac1; RhoA; Cdc42; RHO-GTPASES; IN-VIVO; RECEPTOR; CYTOKINESIS; TRAFFICKING;

D O I：

10.1016/j.febslet.2009.03.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies have identified Rab35 in the endocytic pathway and as a regulator of cytokinesis; however its molecular mechanisms are currently unknown. Here, we find that Rab35 colocalizes with actin. laments and with Cdc42, Rac1 and RhoA, and that Rab35 can activate Cdc42 both in vivo and in vitro. We find activated Rab35 stimulates neurite outgrowth in PC12 and N1E-115 cells via a Cdc42-dependent pathway and that siRNA knockdown of Rab35 activity abolishes neurite outgrowth in these cell lines. We conclude that one function of Rab35 is to regulate Rho-family GTPases and that this role has consequences for neurite outgrowth.

引用

页码：1096 / 1101

页数：6

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