RUST RESISTANCE EVALUATION OF ADVANCED WHEAT (TRITICUM AESTIVUM L.) GENOTYPES USING PCR-BASED DNA MARKERS

被引：0

作者：

Parveen, Zahida ^{[1
]}

Iqbal, Naeem ^{[1
]}

Sajid-Ur-Rahman ^{[2
]}

Younis, Muhammad ^{[2
]}

Nawaz, Muhammad ^{[3
]}

Raza, S. Hammad ^{[1
]}

Iqbal, M. Zaffar ^{[2
]}

机构：

[1] GC Univ, Dept Bot, Plant Physiol Lab, Faisalabad, Pakistan

[2] AARI, Agr Biotechnol Res Inst, Faisalabad, Pakistan

[3] GC Univ, Dept Bioinformat & Biotechnol, Faisalabad, Pakistan

来源：

PAKISTAN JOURNAL OF BOTANY | 2014年 / 46卷 / 01期

关键词：

LEAF RUST; MOLECULAR MARKERS; MICROSATELLITE MARKERS; ASSISTED SELECTION; SSR MARKERS; GENE LR10; IDENTIFICATION; VALIDATION;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The most effective and environmental friendly approach for the control of wheat rust disease is the use of resistant genotypes. The present study was conducted to explore rust resistance potential of 85 elite wheat genotypes (36 varieties and 49 advanced lines) using various types of DNA markers like STS, SCAR and SSR. DNA markers linked with different genes conferring resistance to rusts (Leaf rust=Lr, Yellow rust=Yr and Stem rust=Sr) were employed in this study. A total of 18 genes, consisting of eleven Lr (lr1, lr10, lr19, lr21, lr28, lr34, lr39, lr46, lr47, lr51 and lr52), four Yr (yr5, yr18, yr26 and yr29) and three Sr genes (sr2, sr29, and sr36) were studied through linked DNA markers. Maximum number of Lr genes was found in 17 advanced lines and 9 varieties, Yr genes in 26 advanced lines and 20 wheat varieties, and Sr genes in 43 advanced lines and 27 varieties. Minimum number of Lr genes was found in advanced line D-97 and variety Kohinoor-83, Yr genes in wheat variety Bwp-97 and Sr genes in 6 advanced lines and 8 varieties. Molecular data revealed that genotypes having same origin, from a specified area showed resistance for similar type of genes. In this study, an average similarity of 84% was recorded among wheat genotypes. Out of 18 loci, 15 were found to be polymorphic.

引用

页码：251 / 257

页数：7

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