New Silk Fibroin-Based Bioresorbable Microcarriers

被引：7

作者：

Arkhipova, A. Yu. ^{[1
]}

Kotlyarova, M. C. ^{[1
]}

Novichkova, S. G. ^{[1
]}

Agapova, O. I. ^{[2
]}

Kulikov, D. A. ^{[3
]}

Kulikov, A. V. ^{[4
]}

Drutskaya, M. S. ^{[5
]}

Agapov, I. I. ^{[2
]}

Moisenovich, M. M. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Biol, Moscow, Russia

[2] Minist Hlth Russian Federat, VI Shumakov Fed Res Ctr Transplantol & Artificial, Moscow, Russia

[3] MF Vladimirsky Moscow Reg Res & Clin Inst, Moscow, Russia

[4] Russian Acad Sci, Inst Theoret & Expt Biophys, Moscow, Russia

[5] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2016年 / 160卷 / 04期

关键词：

microcarrier; fibroblasts; fibroin; biopolymers; IN-VITRO; DIFFERENTIATION; EXPANSION; VIVO;

D O I：

10.1007/s10517-016-3204-x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We fabricated bioresorbable microcarriers from water solution of Bombyx mori silk fi broin. The microcarriers are 3D structures with intricate surface and pores allowing penetration of culture medium, gas exchange, and cell adhesion. Fibroin molecules form hydrophobic structures and normally have a negative charge, which stimulates migration, but inhibits cell adhesion and makes it less effective. In order to improve adhesion efficiency and velocity, gelatin (hydrophilic biopolymer with integrin-recognizing RGD sequence) was added to the microcarrier composition. The resultant bioresorbable microcarriers support adhesion and proliferation of 3T3 murine fibroblasts.

引用

页码：491 / 494

页数：4

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