Guidelines for validation of DNA extraction methods applied in subsequent PCR analysis of food and feed products for the presence of genetically modified material

被引：10

作者：

Waiblinger, Hans-Ulrich ^{[1
]}

Grohmann, Lutz ^{[2
]}

机构：

[1] Chem & Vet Untersuchungsamt Freiburg, D-79114 Freiburg, Germany

[2] Bundesamt Verbraucherschutz & Lebensmittelsicherh, D-10117 Berlin, Germany

来源：

JOURNAL FUR VERBRAUCHERSCHUTZ UND LEBENSMITTELSICHERHEIT-JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY | 2014年 / 9卷 / 02期

关键词：

DNA extraction; Validation; In-house; Inter-laboratory study; Real-time PCR; Genetically modified organism (GMO); INTERLABORATORY VALIDATION; IN-HOUSE;

D O I：

10.1007/s00003-014-0862-3

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Guidelines for the validation of DNA extraction methods especially in the scope of the analysis of genetically modified sequences in food and feed products by real-time polymerase chain reaction (real-time PCR) are described. Performance testing of extracted DNA is primarily done by determination of the quantity and quality of amplifiable DNA in real-time PCR. Assessment of quality of extracted DNA includes determination of DNA concentration (e.g. fluorimetrically) and evaluation of fragmentation status by gel electrophoresis. A detailed procedure for in-house validation as well as subsequent inter-laboratory validation studies of DNA extraction methods is presented.

引用

页码：183 / 190

页数：8

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