Mutagenicity in Escherichia coli of the major DNA adduct derived from the endogenous mutagen malondialdehyde

The spectrum of mutations induced by the naturally occurring DNA adduce pyrimido[1,2-alpha]purin-10(3H)-one (M(1)G) was determined by site-specific approaches using M13 vectors replicated in Escherichia coli, Mle was placed at position 6256 in the (-)-strand of M13MB102 by ligating the oligodeoxynucleotide 5'-GGT(M(1)G)TCCG-3' into a gapped-duplex derivative of the vector, Unmodified and M(1)G-modified genomes containing either a cytosine or thymine at position 6256 of the (+)-strand were transformed into repair-proficient and repair-deficient E. coli strains, and base pair substitutions were quantitated by hybridization analysis, Modified genomes containing a cytosine opposite M(1)G resulted in roughly equal numbers of M(1)G-->A and M(1)G-->T mutations with few M(1)G-->C mutations. The total mutation frequency was approximate to 1%, which represents a 500-fold increase in mutations compared with unmodified M13MB102. Transformation of modified genomes containing a thymine opposite M(1)G allowed an estimate to be made of the ability of M(1)G to block replication, The (-)-strand was replicated >80% of the time in the unadducted genome but only 20% of the time when M(1)G was present, Correction of the mutation frequency for the strand bias of replication indicated that the actual frequency of mutations induced by M(1)G was 18%, Experiments using E. coli with different genetic backgrounds indicated that the SOS response enhances the mutagenicity of M(1)G and that M(1)G is a substrate for repair by the nucleotide excision repair complex. These studies indicate that M(1)G, which is present endogenously in DNA of healthy human beings, is a strong block to replication and an efficient premutagenic lesion.