Scavengase p20: A novel family of bacterial antioxidant enzymes

被引：33

作者：

Wan, XY ^{[1
]}

Zhou, Y ^{[1
]}

Yan, ZY ^{[1
]}

Wang, HL ^{[1
]}

Hou, YD ^{[1
]}

Jin, DY ^{[1
]}

机构：

[1] CHINESE ACAD PREVENT MED,NATL KEY LAB MOL BIOL & GENET ENGN,BEIJING 100052,PEOPLES R CHINA

来源：

FEBS LETTERS | 1997年 / 407卷 / 01期

关键词：

scavengase p20; antioxidant enzyme; thioredoxin-dependent peroxidase; Helicobacter pylori; sequence analysis; protein microsequencing;

D O I：

10.1016/S0014-5793(97)00302-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel antioxidant enzyme designated scavengase p20 nas identified in various pathogenic bacteria through database searching for sequences strikingly homologous to a recently discovered Escherichia coli thiol peroxidase p20, The direct biochemical evidence for the existence of scavengase p20 in Haemophilus influenzae, Streptococcus pneumoniae and Helicobacter pylori was provided by protein microsequencing and by in vitro assays for antioxidant activities. Overlapping genes encoding scavengase p20 and superoxide dismutase were recognized in H. pylori and their functional implications are discussed. (C) 1997 Federation of European Biochemical Societies.

引用

页码：32 / 36

页数：5

共 25 条

[1] ISSUES IN SEARCHING MOLECULAR SEQUENCE DATABASES [J].

ALTSCHUL, SF ;

BOGUSKI, MS ;

GISH, W ;

WOOTTON, JC .

NATURE GENETICS, 1994, 6 (02) :119-129

[2] THIOREDOXIN-LINKED THIOL PEROXIDASE FROM PERIPLASMIC SPACE OF ESCHERICHIA-COLI [J].

CHA, MK ;

KIM, HK ;

KIM, IH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (48) :28635-28641

[3] CLONING AND SEQUENCING OF THIOL-SPECIFIC ANTIOXIDANT FROM MAMMALIAN BRAIN - ALKYL HYDROPEROXIDE REDUCTASE AND THIOL-SPECIFIC ANTIOXIDANT DEFINE A LARGE FAMILY OF ANTIOXIDANT ENZYMES [J].

CHAE, HZ ;

ROBISON, K ;

POOLE, LB ;

CHURCH, G ;

STORZ, G ;

RHEE, SG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (15) :7017-7021

[4]

CHAE HZ, 1994, J BIOL CHEM, V269, P27670

[5]

Felsenstein J, 1996, METHOD ENZYMOL, V266, P418

[6] PROGRESSIVE SEQUENCE ALIGNMENT AS A PREREQUISITE TO CORRECT PHYLOGENETIC TREES [J].

FENG, DF ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR EVOLUTION, 1987, 25 (04) :351-360

[7] NUCLEOTIDE-SEQUENCE ANALYSIS OF A TYPE-1 FIMBRIAL GENE OF STREPTOCOCCUS-SANGUIS FW213 [J].

FENNO, JC ;

LEBLANC, DJ ;

FIVESTAYLOR, P .

INFECTION AND IMMUNITY, 1989, 57 (11) :3527-3533

[8] WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD [J].

FLEISCHMANN, RD ;

ADAMS, MD ;

WHITE, O ;

CLAYTON, RA ;

KIRKNESS, EF ;

KERLAVAGE, AR ;

BULT, CJ ;

TOMB, JF ;

DOUGHERTY, BA ;

MERRICK, JM ;

MCKENNEY, K ;

SUTTON, G ;

FITZHUGH, W ;

FIELDS, C ;

GOCAYNE, JD ;

SCOTT, J ;

SHIRLEY, R ;

LIU, LI ;

GLODEK, A ;

KELLEY, JM ;

WEIDMAN, JF ;

PHILLIPS, CA ;

SPRIGGS, T ;

HEDBLOM, E ;

COTTON, MD ;

UTTERBACK, TR ;

HANNA, MC ;

NGUYEN, DT ;

SAUDEK, DM ;

BRANDON, RC ;

FINE, LD ;

FRITCHMAN, JL ;

FUHRMANN, JL ;

GEOGHAGEN, NSM ;

GNEHM, CL ;

MCDONALD, LA ;

SMALL, KV ;

FRASER, CM ;

SMITH, HO ;

VENTER, JC .

SCIENCE, 1995, 269 (5223) :496-512

[9]

FRAZON VL, 1990, INFECT IMMUN, V58, P529

[10] NUCLEOTIDE-SEQUENCE OF A GENE CODING FOR A SALIVA-BINDING PROTEIN (SSAB) FROM STREPTOCOCCUS-SANGUIS 12 AND POSSIBLE ROLE OF THE PROTEIN IN COAGGREGATION WITH ACTINOMYCES [J].

GANESHKUMAR, N ;

HANNAM, PM ;

KOLENBRANDER, PE ;

MCBRIDE, BC .

INFECTION AND IMMUNITY, 1991, 59 (03) :1093-1099

← 1 2 3 →