Purification and enzymatic characterization of two β-endoxylanases from Trichoderma sp K9301 and their actions in xylooligosaccharide production

被引：39

作者：

Chen, Lei-Lei ^{[1
]}

Zhang, Min ^{[1
]}

Zhang, Dong-Hua ^{[1
]}

Chen, Xiu-Lan ^{[1
]}

Sun, Cai-Yun ^{[1
]}

Zhou, Bai-Cheng ^{[1
]}

Zhang, Yu-Zhong ^{[1
]}

机构：

[1] Shandong Univ, Marine Biotechnol Res Ctr, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 21期

关键词：

Trichoderma; Endoxylanase; Xylooligosaccharide; Xylan; Characterization; XYLANASE; FAMILIES; OLIGOSACCHARIDES; HYDROLASES;

D O I：

10.1016/j.biortech.2009.05.038

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Trichoderma sp. K9301 secreting endoxylanases with an activity of 2836 U/g (dry weight) was screened for XOs production. Two acidic beta-endoxylanases EX1 (30.1 kDa) and EX2 (20.1 kDa) were purified from crude extract of the strain K9301 in solid fermentation. Action modes of EX1 and EX2 towards XOs showed similar hydrolysis characters to endoxylanases belonging to glycosyl hydrolase family 10 and 11, respectively. EX1 exhibited better affinity but lower hydrolytic efficiency than EX2 to xylans from beechwood, birchwood, and oat-spelt. They had synergistic action on xylan hydrolysis. The optimum condition to prepare XOs from corncobs was obtained as 10 mg/ml corncob xylan incubated with 10 U/mg crude enzymes at 50 degrees C for 3 h. The yield of XOs reached 43.3%, and only a little amount of xylose (3.1%) was simultaneously produced, suggesting the good potential of strain K9301 in XOs production. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：5230 / 5236

页数：7

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